三个江西不同品种鸡apoA-Ⅰ、apoB基因表达与脂类代谢的相关性分析

发布时间：2018-05-29 04:48

本文选题：鸡 + 载脂蛋白　；参考：《江西农业大学》2015年硕士论文

【摘要】：本试验旨探讨apo A-Ⅰ和apo B m RNA表达水平与脂类代谢的相关性。选取57周龄健康的泰和乌鸡、宁都黄鸡和安义瓦灰鸡,经45天常规饲养后,每个品种随机选出12羽,作为试验动物,取材,并进行鸡蛋胆固醇含量、肝脏粗脂肪含量、血液生化指标以及apo A-Ⅰ和apo B m RNA表达量的测定。试验结果如下:(1)宁都黄鸡肝湿重/活体重、腹脂重和腹脂比率显著高于其它两个品种(P0.05)。(2)宁都黄鸡血清中TC含量显著高于安义瓦灰鸡(P0.05);安义瓦灰鸡血清中LDL含量显著高于宁都黄鸡(P0.05);泰和乌鸡血清中LPS活性显著高于宁都黄鸡;三个品种间血清中TG和HDL含量无显著差异(P0.05)。(3)三个品种间apo A-Ⅰ和apo B基因m RNA的表达量无显著差异(P0.05)。(4)泰和乌鸡apo B基因的m RNA表达量:在肝脏中的表达量与血清中LPS活性具有显著正相关性(r=0.586,P0.05);在小肠中与肝湿重具有显著负相关性(r=-0.545,P0.05)。(5)宁都黄鸡apo A-Ⅰ基因的m RNA表达量:在肝脏中的表达量与血清中TG含量和鸡蛋中胆固醇含量具有显著负相关性(r=-0.583,r=-0.582,P0.05)。(6)安义瓦灰鸡apo A-Ⅰ基因m RNA表达量:在肝脏中的表达量与血清中LPS活性具有显著负相关性(r=-0.583,P0.05),而与肝湿重和腹脂重具有显著正相关性(r=0.591,r=0.649,P0.05);在小肠中与鸡蛋中胆固醇含量和血清中TC含量具有显著负相关性(r=-0.685,r=-0.616,P0.05)。结论:Apo A-Ⅰ基因的m RNA表达量与宁都黄鸡和安义瓦灰鸡的血液及鸡蛋中脂类代谢指标具有显著负相关性,但与安义瓦灰鸡腹脂重和肝湿重具有显著正相关性,与泰和乌鸡的脂类代谢指标无相关性;apo B基因表达量与泰和乌鸡肝湿重具有显著负相关性,与血清中LPS活性具有显著正相关性,与宁都黄鸡和安义瓦灰鸡的脂类代谢指标无相关性。
[Abstract]:The purpose of this study was to investigate the correlation between the expression level of apo A- I and apo B m RNA and the lipid metabolism. The 57 week old Taihe chicken, Ningdu yellow chicken and an Anyi gray chicken were selected for 45 days, and 12 feathers were selected randomly after 45 days of routine feeding. And the determination of the expression of apo A- I and apo B m RNA. The results were as follows: (1) the wet weight / live weight of the Ningdu yellow chicken liver, the abdominal fat weight and the abdominal fat ratio were significantly higher than those of the other two varieties (P0.05). (2) the serum TC content in the serum of Ningdu yellow chicken was significantly higher than that of the yam ash chicken (P0.05); the serum LDL content in the sera of the Yanyu gray chicken was significantly higher than that of Ningdu yellow chicken (P0.05); Thailand. The activity of LPS in sera was significantly higher than that of Ningdu yellow chicken; there was no significant difference in the content of TG and HDL in the serum between three varieties (P0.05). (3) there was no significant difference in the expression of M RNA between the three varieties of apo A- I and apo B gene (P0.05). (4) the expression amount in the liver and the activity of the serum in the liver were significantly positive. Correlation (r=0.586, P0.05); there was a significant negative correlation with the wet weight of the liver in the small intestine (r=-0.545, P0.05). (5) the m RNA expression of the apo A- I gene in Ningdu yellow chicken: the expression in the liver was negatively correlated with the serum TG content and the cholesterol content in the eggs (r=-0.583, r=-0.582, P0.05). (6) Expression amount: there is a significant negative correlation between the expression in the liver and the activity of LPS in the serum (r=-0.583, P0.05), but it has a significant positive correlation with the liver wet weight and abdominal fat weight (r=0.591, r=0.649, P0.05). There is a significant negative correlation between the cholesterol content in the small intestine and the TC content in the serum (r=-0.685, r=-0.616, P0.05). Conclusion: Apo A- I base There was a significant negative correlation between the m RNA expression and the lipid metabolism index in the blood and egg of the Ningdu yellow chicken and the Yanyu gray chicken, but it had a significant positive correlation with the abdominal fat weight and the wet weight of the liver, and had no correlation with the lipid metabolism of the Taihe black chicken. The amount of apo B gene expression was significantly negatively correlated with the liver wet weight of the Taihe black chicken. Sex was positively correlated with LPS activity in serum, but was not correlated with lipid metabolism indexes in Ningdu yellow chicken and Anyi tile gray chicken.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831

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