秦岭部分珍稀动物蓝氏贾第虫、隐孢子虫和毕氏肠微孢子虫种群结构研究

发布时间：2018-05-29 09:01

本文选题：珍稀动物 + 隐孢子虫　；参考：《西北农林科技大学》2015年硕士论文

【摘要】：蓝氏贾第虫(Giardia lamblia)、隐孢子虫(Cryptosporidium spp.)和毕氏肠微孢子虫(Enterocytozoon bieneusi)是3类重要的机会性致病原虫,在经济发达和欠发达地区均广泛流行,对人的健康以及动物的生长发育造成严重的影响,甚至死亡。G.lamblia、Cryptosporidium spp.和E.bieneusi的宿主广泛,可感染人、畜禽和多种野生/圈养珍稀动物。近期的研究表明,珍稀动物在人与动物的感染过程中具有一定的作用,可引发严重的公共卫生隐患。因此,掌握G.lamblia、Cryptosporidium spp.和E.bieneusi的感染情况及种群结构,可为其阻断传播途径、防控其感染提供基础资料。秦岭地区具有丰富的珍稀动植物资源,本研究对陕西秦岭地区部分圈养珍稀动物进行G.lamblia、Cryptosporidium spp.和E.bieneusi感染状况调查,并对所获得的阳性分离株进行多基因位点序列分型。获得以下结果:1.2013年9月至2014年12月,对18个物种590份样品进行蓝氏贾第虫分子检测,总感染率为3.6%(21/590),主要感染羚牛(8.9%,17/191)、猕猴(3.5%,3/86)、松鼠猴(0.5%,1/20)。DNA序列分析发现G.lamblia集聚体E(18/21)和B(3/21)两种集聚体。MLST分析表明,集聚体E具有显著多态性,在SSU rRNA、TPI、BG和GDH位点分别存在2、11、10、7个SNPs,构成15种不同MLGs;而集聚体B仅存在一种MLG。2.选取18S rRNA和COWP基因,对上述18个物种590份样品进行隐孢子虫感染检测,感染率为3.9%(23/590),其中羚牛为7.9%(15/191)、猕猴为9.3%(8/86)。种类鉴定为微小隐孢子虫(3/23)和安氏隐孢子虫(20/23)。GP60基因分析发现微小隐孢子虫具有2种不同的亚型:IId A15G2R1(羚牛源)和IId A19G1(猕猴源)。安氏隐孢子虫具有两种MLST亚型为A1,A4,A4,A1和A4,A4,A4,A1。3.基于ITS基因对590份样品进行E.bieneusi分子检测,阳性率为10.5%(62/590),分布于偶蹄类动物(羚牛、斑羚、麂子、长颈鹿)、灵长类动物(猕猴、金丝猴、博士猴、狒狒、松鼠猴)、食肉目(大熊猫、小熊猫)。共发现13种不同的ITS基因型,其中包括人兽共患性基因型D、D-new、MH和EbpC。MS1、MS3、MS4和MS7基因位点均具有显著多态性,构成16种不同MLGs,其中MLG4同时见于羚牛和大熊猫,MLG2同时见于羚牛和猕猴,其余MLGs均见于单一物种。
[Abstract]:Giardia lamblia, Cryptosporidium spp.. And Enterocytozoon bieneusii) are three important opportunistic protozoa, which are widely prevalent in economically developed and underdeveloped areas, and have serious effects on human health and the growth and development of animals, and even on the death of .G.lambliae Cryptosporidium spp. And E.bieneusi hosts a wide range of humans, livestock and poultry and a variety of wild / captive rare animals. Recent studies have shown that rare animals play a role in the process of human and animal infection, which can lead to serious public health risks. Therefore, to master G. lamblia Cryptosporidium spp. The infection status and population structure of E.bieneusi can provide basic information for its blocking transmission pathway and preventing and controlling its infection. There are abundant rare flora and fauna resources in Qinling area. In this study, G. lamblia Cryptosporidium spp. was carried out in some captive animals in Qinling area, Shaanxi Province. And to investigate the infection status of E.bieneusi, and to type the polygenic loci of the positive isolates. The following results were obtained: 1. From September 2013 to December 2014, 590 samples of 18 species were tested for Giardia lamblia. The total infection rate was 3.610 / 590%, the main infection rate was 8.9% of the antelope, the rhesus macaque 3.55% 86%, the squirrel monkey 0.5% 20% 20% DNA sequence analysis showed that the G.lamblia agglomeration E18 / 21) and the BU 3 / 21) agglomeration. MLST analysis showed that the agglomeration E had significant polymorphism. In the SSU rRNA-TPIBG and GDH sites, there were 21110,7 SNPs, which constituted 15 different MLGs, while agglomeration B contained only one MLG.2. The 18s rRNA and COWP genes were selected to detect Cryptosporidium infection in 590 samples of 18 species mentioned above. The infection rate was 3.9 / 590 / 590 / 590 / 590 / 590 / 590, respectively. The species were identified as Cryptosporidium microspore 3 / 23) and Cryptosporidium Angiosporidium 20 / 23. GP60 gene analysis showed that Cryptosporidium microsporidium had two different subtypes: IId A15G2R1 and IId A19G1. There are two MLST subtypes of Cryptosporidium Ansoni: A1, A4, A1. 3, A4, A1. 3. 590 samples were tested for E.bieneusi based on ITS gene. The positive rate was 10.552 / 590g. It was distributed in cloven-hoofed animals (antelope, pronghorn, muntjac, giraffe, primate (macaque, golden monkey, Ph.D. monkey, baboon), squirrel monkey, carnivorous order (giant panda). Red Panda. A total of 13 different ITS genotypes were found, including the homozoonotic genotypes DHD-newhmh and EbpC.MS1m3MS3MS4 and MS7 gene loci, which formed 16 different ITS genotypes, among which MLG4 was found in both antelope and giant panda ML-G2 in both antelope and rhesus monkey. The rest of MLGs was found in a single species.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.7

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