闽西地区新流行猪伪狂犬病病毒gB基因分子特征分析

发布时间：2018-05-29 11:20

本文选题：猪伪狂犬病病毒 + gB基因　；参考：《中国兽医学报》2017年04期

【摘要】：为了解2013-2015年闽西地区猪伪狂犬病病毒(PRV)流行株的现状、分子生物学特征和遗传演化规律,收集闽西不同地区的24株PRV分离株,并对其gB基因进行遗传变异分析。结果表明:24株PRV分离株与2012年国内不同省份分离的7株PRV变异株核苷酸及氨基酸的同源性较高,分别为99.7%~100.0%和99.3%~100.0%;而与4株国外PRV经典株的核苷酸及氨基酸同源性相对较低,分别为98.3%~98.7%和96.6%~97.7%。氨基酸多序列比对结果发现,24株PRV分离株与7株2012年国内不同省份分离的PRV变异株gB基因氨基酸序列同源性较高,在几个部位均发生了一致性的变化,有很多相同的特征性氨基酸缺失与替换。遗传进化树分析结果表明,24株PRV分离株与7株PRV变异株处于一个相对独立的亚遗传分支,亲缘关系较近。而与Bartha疫苗株等4株国外经典毒株位于不同的大分支中,亲缘关系较远。预测的抗原表位表明PRV闽西分离株与Bartha疫苗株相比发生了抗原漂移,该差异是否预示闽西地区流行株正在向远离疫苗株的方向变异尚需待进一步研究。
[Abstract]:In order to understand the status quo, molecular biological characteristics and genetic evolution of porcine pseudorabies virus (PRV) epidemic strains from 2013 to 2015 in western Fujian, 24 PRV isolates from different regions of western Fujian were collected and their GB gene genetic variation was analyzed. The results showed that the nucleotide and amino acid homology of PRV isolates from 24 strains of PRV was higher than that of 7 strains of PRV mutants isolated from different provinces of China in 2012, which were 99.70.0% and 99.3%, respectively, while the nucleotide and amino acid homology with 4 foreign classical strains of PRV were relatively low. 98.3% and 96.6% respectively. The results of amino acid multiple sequence alignment showed that the amino acid sequence of GB gene of 24 PRV isolates and 7 PRV mutant strains isolated from different provinces in China in 2012 had high homology, and the amino acid sequence was consistent in several parts. There are many identical characteristic amino acid deletions and substitutions. Genetic phylogenetic tree analysis showed that 24 PRV isolates and 7 PRV variants were in a relatively independent subgenetic branch, and the genetic relationship was close. However, four foreign classical strains, such as Bartha vaccine strain, were located in different branches, and were closely related to each other. The predicted epitopes indicated that the PRV isolates from Minxi showed antigen drift compared with the Bartha vaccine strains. Whether the difference indicates that the prevalent strains in the west of Fujian are mutating away from the vaccine strains need to be further studied.
【作者单位】：龙岩学院生命科学学院;福建省生猪疫病防控工程技术研究中心;
【基金】：国家自然科学基金青年科学基金资助项目(31602048) 福建省农业科技重大专项资助项目(2014NZ0002-3) 福建省科技厅自然科学基金面上资助项目(2016J01712) 龙岩学院产学研合作资助项目(LC2015010)
【分类号】：S852.65

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