饲粮硒含量对酮病奶牛氧化应激的缓解作用

发布时间：2018-05-29 15:29

  本文选题：酮病奶牛 + 硒　； 参考：《动物营养学报》2016年12期

【摘要】：本试验旨在通过研究饲粮硒含量对酮病奶牛氧化应激的缓解作用,并确定酮病奶牛对硒的最适需求量。采用完全随机区组设计,将40头胎次为(3.43±0.87)胎、泌乳天数为(6.33±1.14)d、体重为(644±36)kg、平均泌乳量为(38.42±4.82)kg/d、血浆β-羟丁酸(BHBA)水平为(1.50±0.26)mmol/L的荷斯坦奶牛随机分成4组,每组10头牛。第1组为对照组,饲喂基础饲粮(硒含量为0.15 mg/kg DM);第2、3、4组为试验组,在基础饲粮中加入亚硒酸钠(Na2Se O3)至饲粮硒含量分别达到0.30(试验组1)、0.45(试验组2)和0.60 mg/kg DM(试验组3)。试验分为3期,每期1周。试验每期结束后采集血样,并在第2期结束后每组随机选取5头牛采集肝组织。结果表明:1)试验组酮病奶牛全血、肝脏中硒含量,肝脏中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GPx)活性以及GPx1与GPx4mRNA的相对表达水平均显著高于对照组(P0.05),同时试验组2和3还显著高于试验组1(P0.05)。2)试验组酮病奶牛肝脏中丙二醛(MDA)和过氧化氢(H2O2)含量均显著低于对照组(P0.05),同时试验组2和3还显著低于试验组1(P0.05)。3)试验组酮病奶牛血浆非酯化脂肪酸(NEFA)水平显著低于对照组(P0.05),但血浆葡萄糖和BHBA水平与对照组相比无显著差异(P0.05)。4)饲粮硒含量为0.45和0.60 mg/kg DM时,酮病奶牛全血和肝脏中硒含量、肝脏抗氧化指标和血浆能量代谢指标均无显著差异(P0.05)。由此得出,饲粮硒含量为0.45 mg/kg DM时可显著改善酮病奶牛机体的抗氧化能力。
[Abstract]:The aim of this study was to study the effect of dietary selenium on oxidative stress and determine the optimum demand for selenium in ketogenic cows. A complete randomized block design was used to divide 40 Holstein cows with 3.43 卤0.87, 6.33 卤1.14 d, 644 卤36 kg, 38.42 卤4.82 kg / d, and 1.50 卤1.50 卤0.26)mmol/L plasma 尾 -hydroxybutyrate, respectively, into 4 groups, 10 cows in each group were randomly divided into 4 groups, 10 cows in each group were divided into 4 groups, with a mean lactating capacity of 38.42 卤4.82 kg / d and a plasma 尾 -hydroxybutyrate of 1.50 卤0.26)mmol/L. The first group was fed with basic diet (selenium content was 0.15 mg/kg DMN), the second group was treated with sodium selenite (Na2Se O 3), and the dietary selenium content reached 0.30 (test group 1: 0.45 (test group 2) and 0.60 mg/kg DM3 (test group 3). The trial was divided into 3 periods for one week each. Blood samples were collected at the end of each phase, and liver tissues were collected randomly from 5 cattle in each group after the end of phase 2. The results showed that the selenium content in whole blood and liver of ketosis cows in the test group was higher than that in the control group. The activities of T-AOC, SOD, Glutathione peroxidase (Glutathione peroxidase) and the relative expression of GPx1 and GPx4mRNA in liver were significantly higher than those in control group (P 0.05), while the activity of T-AOC, SOD and Glutathione peroxidase in experimental group 2 and 3 were significantly higher than those in control group. (1) the contents of malondialdehyde (MDAA) and hydrogen peroxide (H _ 2O _ 2) in the liver of ketosis cows were significantly lower than those in control group (P 0.05), and the levels of plasma non-esterified fatty acids (NEFAs) were significantly lower in test groups 2 and 3. The content of selenium in diet was 0.45 and 0.60 mg/kg DM, but there was no significant difference in plasma glucose and BHBA levels between the control group and the control group. There was no significant difference in selenium content in whole blood and liver, liver antioxidant index and plasma energy metabolism index in ketosis cows. It was concluded that dietary selenium content of 0.45 mg/kg DM could significantly improve the antioxidant capacity of ketosis cows.
【作者单位】： 江苏农牧科技职业学院宠物科技学院;上海光明荷斯坦牧业有限公司;
【基金】：生鲜牛乳中潜在生物性危害因子的评估研究[沪农科攻字(2013)第3-1号] 江苏高校品牌专业建设工程资助项目(PPZY2015C230)
【分类号】：S858.23
，

本文编号：1951382