绒山羊背最长肌比较转录组分析及肉品质候选基因筛选

发布时间：2018-05-30 03:07

本文选题：绒山羊 + 背最长肌　；参考：《内蒙古农业大学》2015年硕士论文

【摘要】：内蒙古绒山羊肉因味美鲜嫩、易消化、营养价值高以及脂肪和胆固醇含量低等特点深受消费者喜爱。目前,大多数研究学者主要在理化特性方面对肉品质进行评估测定,很少有人利用转录组学技术研究肉品质。针对这一问题,本研究通过RNA-Seq技术对4只内蒙古绒山羊背最长肌转录组文库进行高通量测序和从头组装,通过研究不同年龄和不同性别绒山羊背最长肌间的差异表达基因,筛选出影响绒山羊肉品质的候选基因,再利用David软件对候选基因进行GO功能注释和KEGG富集分析,从而为绒山羊肉品质相关基因的筛选和功能基因组学的研究提供理论依据。主要研究结果如下：1、成功地构建了4只内蒙古绒山羊背最长肌转录组的样本文库,基于RNA-Seq高通量测序技术,共获得16GB的背最长肌表达谱数据。组装共获得52,415,590条reads,共包含4,717,403,100个核苷酸序列信息,利用De novo方法对reads进行拼接共得到65,329个contigs,N50值为1,059nt。2、通过CLC Genomics Workbench 6.0软件共获得474个差异表达基因。其中成年公羊与成年母羊背最长肌间共有201个差异表达基因；成年公羊与断奶公羔背最长肌间共有120个差异表达基因；成年母羊与断奶母羔背最长肌间共有100个差异表达基因：断奶公羔与断奶母羔背最长肌间共有53个差异表达基因。3、共筛选出263个对绒山羊肉品质有影响的候选基因,其中包含123个高表达有利基因和140个高表达有害基因。4、候选基因GO功能注释结果表明：高表达有利基因主要与骨骼肌的生长发育、细胞器形成和蛋白结合功能相关；高表达有害基因主要与脂质代谢、细胞骨架和结合功能相关。5、候选基因KEGG通路富集分析结果表明：高表达有利基因主要参与糖酵解/糖异生和丝裂原活化蛋白激酶(MAPK)等信号通路,通路中的基因在骨骼肌的生长发育过程中扮演着重要的角色,但在骨骼肌中的作用未被报道；高表达有害基因主要参与凝血-补体级联反应、朊病毒病、ErbB受体、色氨酸代谢和系统性红斑狼疮等信号通路。6、建立了一种筛选绒山羊肉品质候选基因的方法。PDK4、ADIPOQ、CD36、 ADIPOR2和LPIN1等五个基因能够作为绒山羊肉品质重要的候选基因。
[Abstract]:The meat of Inner Mongolia Cashmere Goat is popular with consumers because of its delicious taste, easy digestion, high nutritional value and low fat and cholesterol content. At present, most researchers mainly evaluate meat quality in physical and chemical properties, and few people use transcriptome technology to study meat quality. In order to solve this problem, the high throughput sequencing and ab initio assembly of the longest dorsal transcriptional library of 4 Inner Mongolia Cashmere Goats were carried out by RNA-Seq, and the differentially expressed genes among the longissimus dorsi muscles of different ages and different genders were studied. Candidate genes affecting the meat quality of cashmere goats were screened, and then go functional annotation and KEGG enrichment analysis of candidate genes were carried out by David software, which provided theoretical basis for screening genes related to meat quality and functional genomics of cashmere goats. The main results are as follows: 1. We successfully constructed a sample library of the longest dorsal muscle transcriptome of four Inner Mongolia cashmere goats. Based on the RNA-Seq high-throughput sequencing technique, we obtained the expression profile data of the longissimus dorsi of 16GB. A total of 52415590 RDS were obtained, containing 4717403100 nucleotide sequence information. 65329 contigsN50 values of 1059nt.2were obtained by de novo method. A total of 474 differentially expressed genes were obtained by CLC Genomics Workbench 6.0 software. There were 201 differentially expressed genes in the longissimus dorsi muscle between adult ram and adult ewe, 120 differentially expressed genes in the longissimus dorsi muscle of adult ram and weaning male lamb. There were 100 differentially expressed genes between the longissimus muscle of weaning ewe and weaning female lambs: 53 differentially expressed genes between the longissimus muscle of weaning male lambs and weaning female lambs. 263 candidate genes affecting the meat quality of cashmere goats were screened out. There were 123 highly expressed favorable genes and 140 highly expressed harmful genes. The results of functional annotation of candidate gene go showed that the high expression favorable genes were mainly related to skeletal muscle growth, organelle formation and protein binding function. Overexpression of harmful genes is mainly associated with lipid metabolism, Cytoskeleton and binding function were related to. 5. The results of enrichment analysis of candidate gene KEGG pathway showed that overexpression of favorable genes was mainly involved in glycolytic / glycolytic and mitogen-activated protein kinase (mitogen-activated protein kinase) signaling pathways. The gene in the pathway plays an important role in the growth and development of skeletal muscle, but its role in skeletal muscle is not reported. The high expression of harmful genes is mainly involved in the clotting complement cascade reaction, prion disease and ErbB receptor. Tryptophan metabolism and systemic lupus erythematosus (SLE) signaling pathway. 6. A method for screening candidate genes of Cashmere Goat meat quality, including CD36, ADIPOR2 and LPIN1, was established, which can be used as an important candidate gene for Cashmere Goat meat quality.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S827

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