褪黑素对热应激猪精子质量的影响

发布时间：2018-05-31 00:40

本文选题：精子质量 + 褪黑素　；参考：《吉林农业大学学报》2016年06期

【摘要】：为研究褪黑素(Melatonin,MLT)对热应激引起的猪精子质量下降的缓解作用及其机制。首先用免疫荧光方法检测MLT受体在猪精子上的存在和分布;然后将稀释后的精子分为对照组(ck)、热应激组(H)、MLT组(M)、热应激+MLT组(H+M)、热应激+MLT受体拮抗剂Luzindole组(H+L)和热应激+MLT受体拮抗剂Luzindole+MLT组(H+L+M)6个组,处理结束后检测各组精子活率、活力、线粒体活性、钙离子水平以及顶体完整率。结果显示:猪精子中存在MLT受体MT1和MT2,且均分布于精子头部;与对照组相比热应激组各项指标显著下降,MLT组精子活率没有显著改变(P0.05),但精子活力、线粒体活性、钙离子水平和顶体完整率显著升高(P0.05)。添加MLT受体拮抗剂Luzindole后精子活力、线粒体活性和钙离子水平显著下降(P0.05),顶体完整率呈一定程度下降,但差异不显著(P0.05)。以上结果表明:热应激会导致猪精子质量显著下降,并且MLT能通过受体途径改善热应激对猪精子质量的影响,但同时也存在非受体介导的作用。
[Abstract]:To study the effect of melatonin in MLT on the reduction of sperm quality induced by heat stress and its mechanism. Firstly, the existence and distribution of MLT receptor on pig sperm were detected by immunofluorescence method. The diluted spermatozoa were divided into six groups: control group, heat stress MLT receptor antagonist Luzindole group, and heat stress MLT receptor antagonist Luzindole MLT group. Sperm motility, mitochondrial activity, calcium level and acrosome integrity were measured after treatment. The results showed that there were MLT receptors MT1 and MT2 in porcine spermatozoa, which were distributed in the head of spermatozoa, and the sperm motility and mitochondrial activity in heat stress group were not significantly changed compared with those in control group, but the motility of spermatozoa was not significantly changed in heat stress group. Calcium ion level and acrosome integrity rate increased significantly (P 0.05). After the addition of MLT receptor antagonist Luzindole, sperm motility, mitochondrial activity and Ca ~ (2 +) level decreased significantly, and the acrosome integrity rate decreased to a certain extent, but the difference was not significant (P 0.05). These results suggest that heat stress can significantly decrease the sperm quality of pigs and that MLT can improve the effect of heat stress on the quality of pig spermatozoa through the receptor pathway, but there is also a non-receptor-mediated effect.
【作者单位】：西藏自治区农牧科学院畜牧兽医研究所;
【基金】：西藏自治区财政专项[藏财预指(2016)01号]
【分类号】：S828

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