pagP基因对禽致病性大肠杆菌抗菌肽抗性和致病性的影响

发布时间：2018-05-31 01:26

本文选题：禽致病性大肠杆菌 + pagP基因　；参考：《微生物学通报》2017年12期

【摘要】：【目的】禽致病性大肠杆菌(Avian pathogenic Escherichia coli,APEC)不仅严重影响全球的养禽业,对人类公共健康也造成巨大的潜在威胁。pag P基因在细菌的抗菌肽抗性和致病性方面发挥重要作用,但关于pag P基因在APEC中的功能尚不清楚。本文构建禽致病性大肠杆菌pag P基因缺失株,对缺失株的抗菌肽抗性和致病性进行研究。【方法】利用Red重组系统构建APEC的pag P基因缺失株,然后利用回复质粒构建回复株。研究pag P基因对细胞黏附与入侵、生物被膜形成能力、外膜渗透性、抗菌肽敏感性、致病性等方面的影响。【结果】成功构建pag P基因缺失株和回复株,抗菌肽抗性试验发现pag P基因缺失株对多粘菌素B、鸡β-防御素2(AVBD2)的敏感性显著增加(P0.01),致病性试验结果表明pag P基因缺失株的毒力显著降低(P0.01)。【结论】APEC的pag P基因对AVBD2的敏感性和APEC的致病性密切相关,为深入研究pag P基因的功能及调控作用奠定了基础。
[Abstract]:[objective] avian pathogenic Escherichia coli (Avian pathogenic Escherichia coli) not only seriously affects poultry industry worldwide, but also poses a great potential threat to human public health. Pak P gene plays an important role in bacterial antimicrobial peptide resistance and pathogenicity. However, the function of pag P gene in APEC is unclear. In this paper, the pag P gene deletion strain of avian pathogenic Escherichia coli was constructed, and the antimicrobial peptide resistance and pathogenicity of the deletion strain were studied. [methods] the pag P gene deletion strain of APEC was constructed by Red recombination system, and then the pag P gene deletion strain was constructed by reverse-plasmid. The effects of pag P gene on cell adhesion and invasion, biofilm formation, outer membrane permeability, antimicrobial peptide sensitivity and pathogenicity were studied. Antimicrobial peptide resistance test showed that the sensitivity of pag P gene deletion strain to polymyxin B and chicken 尾 -defensin 2 / AVBD2) increased significantly. The pathogenicity test showed that the virulence of pag P gene deletion strain was significantly lower than that of pag P gene deletion strain. [conclusion] the pag P-base of APEC was significantly decreased. The sensitivity to AVBD2 is closely related to the pathogenicity of APEC. It lays a foundation for further study on the function and regulation of pag P gene.
【作者单位】：安徽农业大学兽医病理生物学与疫病防控安徽省重点实验室;
【基金】：国家自然科学基金项目(No.31372402) 安徽农业大学引进与稳定人才项目(No.yj2017-31)~~
【分类号】：S852.612

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