蛋氨酸缺乏对蛋鸡产蛋后期生产性能、血清游离氨基酸含量和肝脏蛋氨酸代谢相关基因表达的影响

发布时间：2018-06-01 21:16

本文选题：蛋氨酸 + 蛋鸡　；参考：《动物营养学报》2017年09期

【摘要】：本试验旨在研究蛋氨酸缺乏对蛋鸡产蛋后期生产性能、血清游离氨基酸含量和肝脏蛋氨酸代谢相关酶基因表达的影响。选取180只62周龄的海兰灰蛋鸡,根据产蛋率均匀分成3组(每组6个重复,每个重复10只):对照组饲喂蛋氨酸水平为0.33%的饲粮,蛋氨酸缺乏组分别饲喂蛋氨酸水平为0.21%和0.27%的饲粮,试验期90d。结果表明:1)饲粮蛋氨酸缺乏显著降低了蛋鸡的平均日采食量、产蛋率、平均日产蛋重、平均蛋重和不合格蛋率(P0.05),显著提高了料蛋比(P0.05)。2)蛋氨酸缺乏显著降低蛋鸡血清中蛋氨酸的含量(P0.05)。0.21%蛋氨酸组蛋鸡血清中丝氨酸、甘氨酸和丙氨酸的含量显著高于其他2组(P0.05)。0.27%蛋氨酸组蛋鸡血清中缬氨酸、异亮氨酸和精氨酸的含量显著低于对照组(P0.05),脯氨酸的含量显著低于0.21%蛋氨酸组(P0.05)。3)与对照组相比,0.21%蛋氨酸组蛋鸡肝脏中DNA甲基转移酶1、N6-甲基腺苷(m6 A)甲基转移酶3(METTL3)和m6 A甲基转移酶14的表达量显著提高(P0.05),0.27%蛋氨酸组蛋鸡肝脏中METTL3的表达量显著提高(P0.05)。4)与对照组相比,0.21%蛋氨酸组蛋鸡肝脏中甲硫氨酸腺苷转移酶1a、5-甲基四氢叶酸-同型半胱氨酸甲基转移酶(MTR)和胱硫醚-β-合成酶的表达量显著提高(P0.05),0.27%蛋氨酸组蛋鸡肝脏中MTR的表达量显著提高(P0.05)。蛋氨酸缺乏对蛋鸡肝脏甲硫氨酸腺苷高半胱氨酸酶和甜菜碱高半胱氨酸甲基转移酶的表达量无显著影响(P0.05)。由以上结果可知:蛋鸡饲粮高水平的蛋氨酸缺乏会降低蛋鸡的生产性能,可能与蛋氨酸缺乏改变蛋氨酸代谢途径,影响DNA和RNA的甲基化过程有关。
[Abstract]:The purpose of this study was to study the effects of methionine deficiency on the performance of laying hens, the content of serum free amino acids and the expression of enzymes related to methionine metabolism in liver. 180 Hailan gray laying hens aged 62 weeks were divided into 3 groups according to laying rate (6 replicates in each group, 10 in each replica). The control group was fed a diet of 0.33% methionine level. Methionine deficiency group was fed with 0.21% and 0.27% methionine levels for 90 days. The results showed that dietary methionine deficiency significantly decreased the average daily feed intake, laying rate and average daily egg weight of laying hens. The average egg weight and unqualified egg rate (P0.05N) significantly increased the ratio of feed to egg (P0.05U. 2) Met deficiency significantly decreased the content of methionine in the serum of laying hens and the serine content in the serum of laying hens was significantly decreased by P0.05- 0.21% methionine. The contents of glycine and alanine were significantly higher than those in the other two groups. The contents of isoleucine and arginine were significantly lower than those of the control group (P0.05A and proline) the content of proline was significantly lower than that of 0.21% methionine group (P0.05. 3) compared with the control group, the content of DNA methyltransferase (1N6-methyladenosine) methyltransferase (3METTL3) in the liver of laying hens with 0.21% methionine was significantly lower than that in the control group. The expression of m6A methyltransferase 14 and m6A methyltransferase 14 significantly increased the expression of METTL3 in the liver of laying hens with 0.27% methionine.) compared with the control group, the expression of methionine adenosyltransferase 1 aa5-methyltetrahydrofolate in the liver of 0.21% methionine group was significantly higher than that of the control group. The expression of homocysteine methyltransferase (MTR-) and cystathithione- 尾-synthase (P0.05- 尾-synthase) increased significantly in the liver of laying hens treated with 0.27% methionine. Methionine deficiency had no significant effect on the expression of methionine adenosine homocysteinase and betaine homocysteine methyltransferase in the liver of laying hens. The results showed that the high level of methionine deficiency in laying diet could decrease the performance of laying hens, which might be related to the effect of methionine deficiency on the metabolic pathway of methionine and the methylation of DNA and RNA.
【作者单位】：河南科技大学动物科技学院河南省饲草饲料资源开发与畜禽健康养殖院士工作站;中国科学院亚热带农业生态研究所中国科学院亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术研究中心;
【基金】：国家重点研发计划(2016YFD0500504)
【分类号】：S831.5

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