胸膜肺炎放线杆菌血清7型菌株体内外培养转录差异的研究

发布时间：2018-06-02 04:27

本文选题：胸膜肺炎放线杆菌 + 转录组学　；参考：《中国兽医科学》2017年03期

【摘要】：为揭示胸膜肺炎放线杆菌血清7型菌株在体内外培养转录的差异,将该菌培养至对数期采用滴鼻的方式攻毒SPF级昆明小鼠,取小鼠肺组织及菌体纯培养物采用Illumina Hi Seq2500高通量测序平台对该菌在体内外培养的转录差异进行转录组测序(RNA-Seq)分析。结果显示,小鼠肺组织中获取该菌的Clean reads与指定参考基因组的比对效率达15.68%,体外培养菌体的比对效率为97.99%;以后者转录组为对照,共筛选出差异表达基因(DEG)333个,其中上调基因113个,下调基因220个,挖掘新基因6个。对DEG进行GO、COG及KEGG等分析可见,DEG主要富集在能量的产生与转化、碳水化合物转运和代谢、无机离子转运与代谢、氨基酸转运与代谢等方面,主要集中在碳代谢、ATP结合蛋白转运蛋白、嘌呤代谢、氨基酸的生物合成、硫代谢等通路上。本研究对胸膜肺炎放线杆菌血清7型菌株在SPF昆明小鼠体内及体外培养转录差异进行了分析,一定程度上揭示了该菌的致病机制,为后续胸膜肺炎放线杆菌血清7型菌株致病机制的研究提供了依据。
[Abstract]:In order to reveal the transcriptional difference of Actinobacillus pleuropneumoniae serotype 7 strain cultured in vitro and in vivo, the strain was incubated to SPF grade Kunming mice by nasal drip in logarithmic phase. Illumina Hi Seq2500 high-throughput sequencing platform was used to analyze the transcriptional differences in vitro and in vivo. The results showed that the comparison efficiency of the Clean reads obtained from the mouse lung tissue with the specific reference genome was 15.68, and the comparison efficiency of the bacteria cultured in vitro was 97.99%, and 333 differentially expressed genes were screened out using the latter transcriptome as the control. There were 113 up-regulated genes, 220 down-regulated genes and 6 new genes. The analysis of DEG by Gocog and KEGG shows that it is mainly concentrated in energy generation and transformation, carbohydrate transport and metabolism, inorganic ion transport and metabolism, amino acid transport and metabolism, etc. It mainly focuses on carbon metabolism, ATP binding protein transporter, purine metabolism, amino acid biosynthesis, sulfur metabolism and so on. In this study, the transcriptional differences of Actinobacillus pleuropneumoniae serotype 7 in SPF Kunming mice were analyzed in vivo and in vitro, and the pathogenicity of Actinobacillus pleuropneumoniae was revealed to some extent. It provides a basis for the study of pathogenic mechanism of Actinobacillus pleuropneumoniae serotype 7 strain.
【作者单位】：四川农业大学动物医学院动物生物技术中心;动物疫病与人类健康四川省重点实验室;
【基金】：四川省科技支撑计划项目(2014NZ0043) 教育部“长江学者与创新团队发展计划”项目(I RT13083) 四川省十三五育种攻关项目(2016NYZ0052) 国家“十二五”科技支撑计划(2015BAD12B04-2.3)
【分类号】：S852.61

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