猪链球菌2型调控因子Rex缺失株的构建及其致病性鉴定

发布时间：2018-06-02 09:30

本文选题：猪链球菌 + Rex　；参考：《中国预防兽医学报》2017年03期

【摘要】：为鉴定猪链球菌2型强毒株SS2-1调控因子Rex基因的缺失对细菌毒力的影响,本研究利用同源重组方法构建猪链球菌Rex缺失株,命名为SS2-1△Rex,并通过缺失株的生物学特性及其小鼠致病性试验进行鉴定。结果显示亲本株、缺失株及互补株在溶血能力、增殖特性和革兰染色形态无显著差异;在对小鼠致病性试验中,接种SS2-1组小鼠死亡率为87.5%,而SS2-1△Rex组死亡率为12.5%,SS2-1△Rex/p Rex对小鼠的毒力有所恢复,死亡率为50%;Log-Rank(Mantel-Cox)对数秩检验分析结果显示亲本株和缺失株对小鼠致病性存在显著差异。本研究结果表明调节因子Rex是猪链球菌2型的毒力相关因子。本实验为深入研究Rex调控毒力因子的转录和表达及其具体的机制奠定了实验基础。
[Abstract]:In order to identify the effect of the deletion of the SS2-1 regulator Rex gene of Streptococcus suis type 2 strain on the bacterial virulence, the homologous recombination method was used to construct the Rex deletion strain of Streptococcus suis, named SS2-1 Delta Rex, and identified by the biological characteristics of the missing strains and the pathogenicity test of the mice. The results showed the parent strain, the missing strain and the complementary strain. There was no significant difference in hemolysis, proliferation and Gram staining. In the mice inoculated with SS2-1, the mortality of the SS2-1 group was 87.5%, the mortality of the SS2-1 Delta Rex group was 12.5%, the toxicity of SS2-1 Delta Rex/p Rex was recovered and the mortality rate was 50%. The logarithmic rank test of Log-Rank (Mantel-Cox) showed the parent strain and the result of the logarithmic rank test of Log-Rank (Mantel-Cox). The results showed that the regulatory factor Rex is a virulence related factor of Streptococcus suis type 2. This experiment provides an experimental basis for the in-depth study of Rex regulation of the transcription and expression of virulence factors and their specific mechanisms.
【作者单位】：南京农业大学动物医学院;江苏省农业科学院兽医研究所;江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地;
【基金】：国家转基因生物新品种培育重大专项子课题(2014ZX0800946B-002) 国家公益性行业(农业)科研专项子课题(201303041)
【分类号】：S852.611

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