不同添加量的纤维素酶和木聚糖酶对玉米秸秆黄贮的影响

发布时间：2018-06-02 16:27

本文选题：纤维素酶 + 木聚糖酶　；参考：《山西农业大学》2015年硕士论文

【摘要】：本试验采用袋装黄贮方式,分别添加不同量纤维素酶、木聚糖酶进行玉米秸秆黄贮,在10天、30天、50天后开袋测样,筛选出纤维素酶、木聚糖酶的最佳添加量,并确定发酵时间。然后分四组进行黄贮(添加最适纤维素酶组、添加最适木聚糖酶组、混合酶组、对照组),通过CNCPS体系和DFCCS型双外流动态瘤胃培养系统评定不同酶处理的黄贮秸秆对人工瘤胃及其消化率的影响,选出最佳添加组。试验共分三部分：第一部分：对玉米秸秆进行自然条件下风干处理并粉碎,在实验室条件下进行黄贮,分别添加纤维素酶组(添加量为：0,5,10,15,20,25,30ECU/g).木聚糖酶组(添加量为：0,0.05,0.10,0.15,0.20,0.25,0.30IU/g),每个处理设3个重复,常温下贮存10 d、30d、50d后,开封测定黄贮的pH、氨态氮、乳酸、常规养分等。结果表明：添加不同水平的纤维素酶、木聚糖酶对黄贮玉米秸秆的氨态氮占总氮比值(NH3-N/TN)、中性洗涤纤维(NDF)含量的影响差异显著(P0.05),pH值随纤维素酶和木聚糖酶添加量的增加而不断降低,乳酸含量随纤维素酶和木聚糖酶添加量的增加而增加,NDF含量呈下降趋势,且组间差异显著(P0.05),ADF差异不显著(P.0.05),综合感官鉴定和各项指标,纤维素酶的最佳添加量为20ECU/g (DM),木聚糖组最佳添加量是0.2IU/g (DM),最佳黄贮时间为30天。第二部分：将黄贮玉米秸秆分为四组,纤维素酶组(20ECU/g)、木聚糖酶组(0.2IU/g)、纤维素酶(20ECU/g)和木聚糖酶(0.2IU/g)混合组、对照组。常温下贮存30d后,开封测样。用试验室黄贮评价法对其评定,运用CNCPS体系对四组玉米秸秆进行评价。结果表明：各组氨态氮占总氮比值(NH3-N/TN)、乳酸含量差异显著(P0.05),pH值差异不显著(P0.05)。添加混合酶组的LA、CP、PB1、CB1、CC含量均最高,其次为纤维素酶组、木聚糖酶组,对照组含量最低。综合各项指标,黄贮效果最好的为混合酶组,然后是纤维素酶组、木聚糖酶组,最后是对照组。第三部分：将试验二不同组玉米秸秆样品和精料制粒,分为A组(纤维素酶秸秆制粒组)、B组(木聚糖酶秸秆制粒组)、AB组(混合酶秸秆制粒组)和C组(对照组)。利用DFCCS型双外流动态瘤胃培养系统,对A、B、AB、C四组进行仿牛瘤胃发酵处理试验,评价其对人工瘤胃发酵的影响。结果表明：四组中的pH值变化不显著(P0.05), A, B, AB组的表观消化率和总酸均比对照组均高,且组间差异显著(P0.05),AB组最高,A组次之,B组最低。
[Abstract]:In this experiment, different cellulase and xylanase were added to yellow silage by adding different amounts of cellulase and xylanase. The optimum adding amount of cellulase, xylanase and fermentation time were screened at 10 days, 30 days and 50 days later. Then four groups were added to the Yellow silase group (adding the optimum cellulase group and adding the optimum xylanase group). The effects of different enzyme treated yellow silage straw on the artificial rumen and its digestibility were evaluated by CNCPS system and DFCCS dual flow dynamic rumen culture system. The best addition group was selected. The experiment was divided into three parts: the first part: the wind dry treatment and crushing of corn stalk under natural conditions and the laboratory conditions. Under the Yellow silage, the cellulase group was added (adding amount of 0,5,10,15,20,25,30ECU/g). The xylanase group (adding amount was 0,0.05,0.10,0.15,0.20,0.25,0.30IU/g), each treatment was set up 3 repetitions, and stored at 10 d at normal temperature, 30d, and 50D, the pH, ammonia nitrogen, lactic acid and conventional nutrients were determined by Kaifeng, and the results showed that the addition of different levels was added. The effect of Xylanase on the ratio of ammonia nitrogen to total nitrogen (NH3-N/TN) and the content of neutral detergent fiber (NDF) was significantly different (P0.05). The pH value decreased with the increase of cellulase and xylanase, and the content of lactic acid increased with the increase of fibrin and xylanase, and the content of NDF was lower. The difference was significant (P0.05), and the difference of ADF was not significant (P.0.05). The optimum addition amount of cellulase was 20ECU/g (DM), the optimum addition of xylan group was 0.2IU/g (DM) and the best yellow storage time was 30 days. Second parts: the Yellow silage corn straw was divided into four groups, cellulase group (20ECU/g), xylan Enzyme group (0.2IU/g), cellulase (20ECU/g) and xylanase (0.2IU/g) mixed group, control group. After storage of 30d at normal temperature, Kaifeng sample was measured. Four groups of corn stalks were evaluated by test room yellow storage evaluation method and CNCPS system was used to evaluate the ratio of ammonia nitrogen to total nitrogen ratio (NH3-N/TN), and the difference of lactic acid content was significant (P0.05), pH The value difference was not significant (P0.05). The content of LA, CP, PB1, CB1 and CC in the mixed enzyme group was the highest, followed by cellulase group, xylanase group, and the control group with the lowest content. The comprehensive index, the best yellow storage effect was the mixed enzyme group, and then the cellulase group, the xylanase group and the control group. The third part: the experiment two different groups of jade will be tested. Rice straw samples and fine grain granulation were divided into A group (cellulase straw granulating group), B group (xylanase straw granulation group), AB group (mixed enzyme straw granulation group) and C group (control group). The fermentation process of A, B, AB and C four groups was tested by DFCCS double flow dynamic rumen culture system, and the effects on Rumen Fermentation of artificial rumen were evaluated. The results showed that the change of pH value in the four groups was not significant (P0.05). The apparent digestibility and total acid in the group of A, B and AB were all higher than those of the control group, and the difference between the groups was significant (P0.05), the AB group was the highest, the A group was the next, and the B group was the lowest.
【学位授予单位】：山西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S816

【参考文献】