我国部分地区牛源金黄色葡萄球菌基因多态性分型研究

发布时间：2018-06-03 08:21

本文选题：奶牛乳房炎 + 金黄色葡萄球菌　；参考：《中国预防兽医学报》2017年09期

【摘要】：为了解我国牛源金黄色葡萄球菌(S.aureus)的基因多态性,本研究利用随机引物多态性扩增(RAPD)体系对174株分离自贵州、内蒙古、四川、上海、甘肃等地奶牛乳房炎的S.aureus及一株标准菌CVCC2246进行基因分型。结果表明,175株S.aureus均得到清晰的RAPD指纹图谱,扩增产物为1~9个片段,产物大小为240 bp~4 500 bp。所有菌株共分为8个基因型,其中1型16株;2型和3型各37株;4型15株;5型18株;6型14株;7型13株;8型8株。2型和3型菌株占总菌株42%以上,在贵州、内蒙古、四川、上海、甘肃分布广泛,为流行优势基因型;但5型是内蒙古地区的流行优势基因型。各地区菌株基因型比例有明显差异,可能与奶牛饲养水平和环境差异有关。
[Abstract]:In order to understand the genetic polymorphism of S.aureus, 174 strains isolated from Guizhou, Inner Mongolia, Sichuan and Shanghai were amplified by random primer polymorphism (RAPD) system. S.aureus and a standard strain of CVCC2246 were genotyped from dairy cow mastitis in Gansu and other places. The results showed that the RAPD fingerprints of all the S.aureus strains were clear, the amplified products were 1 ~ 9 fragments and the size of the products was 240 bp~4 500 BP. All the strains were divided into 8 genotypes. Among them, 16 strains of type 1 and 37 strains of type 3, type 4, 15 strains, type 5, type 15, type 6, type 14, type 7, type 13, type 8, type 2, and type 3, accounted for more than 42% of the total strains, in Guizhou, Inner Mongolia, Sichuan, Shanghai, China. Gansu province is widely distributed and is the dominant genotype, but type 5 is the dominant genotype in Inner Mongolia. There were significant differences in genotype proportion among different regions, which may be related to the feeding level and environment difference of dairy cattle.
【作者单位】：中国农业科学院兰州畜牧与兽药研究所中国农业科学院新兽药工程重点开放实验室/甘肃省新兽药工程重点实验室;西北民族大学生命科学与工程学院;甘肃农业大学动物医学学院;
【基金】：中央级公益性科研院所基本科研业务费专项资金项目-奶牛养殖环境中耐药数据库及奶牛乳房炎病原菌种库建设(610322016014) 国家重点研发计划“畜禽重要病原耐药性检测与控制技术研究”(2016YFD0501306)
【分类号】：S852.611

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