河南省地方品种鸡白血病净化材料的选择

发布时间：2018-06-04 10:52

本文选题：地方品种鸡 + 禽白血病病毒　；参考：《畜牧与兽医》2017年12期

【摘要】：为了探讨不同材料对禽白血病病毒(ALV)p27抗原检测结果的影响,选择168日龄河南省地方品种母鸡,首先用ELISA检测泄殖腔棉拭子ALV-p27抗原,然后选取部分阳性鸡和阴性鸡分别作为阳性(P)组和阴性(N)组检测其血清和蛋清中ALV-p27抗原,并进行外源性ALV的分离鉴定。结果显示P组蛋清和血清ALV-p27抗原阳性率分别为6304%和7337%;N组蛋清ALV-p27抗原全为阴性,血清ALV-p27抗原阳性率为3315%。P组外源性ALV的分离阳性率为6875%,N组外源性ALV的分离阳性率为278%;蛋清ALV-p27抗原阳性鸡外源性ALV分离阳性率为100%,抗原阴性鸡外源性ALV的分离阳性率为10%;血清ALV-p27抗原阳性鸡外源性ALV的分离阳性率为5946%,抗原阴性鸡外源性ALV的分离阳性率为322%。结果表明,若地方品种鸡群数量较大,育种淘汰率高,采用泄殖腔棉拭子作为检测材料比蛋清检测材料阳性鸡淘汰更彻底;若种鸡群数量较少,淘汰较多影响育种工作,采用蛋清作为检测材料更为合适。
[Abstract]:In order to study the effect of different materials on the detection results of ALV p27 antigen of avian leukemia virus, 168-day-old local hens of Henan Province were selected. The ALV-p27 antigen of cloacal cotton swabs was detected by ELISA. Then some positive and negative chickens were selected as positive and negative groups respectively to detect ALV-p27 antigen in serum and egg white, and to isolate and identify exogenous ALV. The results showed that the positive rates of egg white and serum ALV-p27 antigen in group P and group N were 6304% and 7337%, respectively. The positive rate of serum ALV-p27 antigen was 3315.P, the positive rate of exogenous ALV isolation was 68755.The positive rate of exogenous ALV isolation was 278in N group, the positive rate of exogenous ALV isolation was 100cm in laying white ALV-p27 antigen-positive chickens, and the positive rate of exogenous ALV isolation in egg white ALV-p27 antigen positive chickens was 100%. The isolation positive rate of exogenous ALV in serum ALV-p27 antigen positive chicken was 59466.The positive rate of antigen negative chicken exogenous ALV isolation was 32222. The results showed that if the number of local breeds was large and the breeding elimination rate was high, using cloacal cotton swab as detection material was more thorough than that of egg white positive chicken, and if the number of breeding chickens was small, more elimination would affect the breeding work. It is more suitable to use egg white as testing material.
【作者单位】：河南牧业经济学院动物科技学院;
【基金】：河南省重大科技专项计划项目(161100510200) 校科技创新团队项目(HUAHE2015003)
【分类号】：S858.31

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