一株H6N6亚型野鸟禽流感病毒全基因组序列分析以及对小鼠的感染性研究

发布时间：2018-06-04 13:24

本文选题：禽流感病毒 + HN　；参考：《中国预防兽医学报》2017年06期

【摘要】：为进一步了解2015年分离于我国新疆野鸟粪便中的一株H6N6亚型禽流感病毒(AIV)A/WB/XJ/S1541/2015(H6N6)的生物学特性,本研究对其进行了全基因组测序、遗传演化分析以及BALB/c小鼠的感染性试验。序列分析结果显示:该分离株HA蛋白裂解位点的氨基酸序列为~(339)PQIETR↓GL~(346),仅有1个碱性氨基酸,具有低致病性AIV的分子特征。此外,分离株表面基因HA、NA均与一株H6N6亚型猪流感病毒A/swine/Guangdong/K6/2010高度同源,而内部基因均与H6N6亚型AIV A/pigeon/Guangxi/161/2014(H6N6)高度同源,提示该分离株基因来源的多样性。小鼠的感染性试验结果显示:该病毒可在小鼠的肺脏和鼻甲中有效复制,病毒滴度分别为5.33 log_(10) EID_(50)/mL、4.50 log10EID50/m L,提示其具有感染哺乳动物的潜在风险。本研究结果为H6亚型AIV的持续性监测和相关生物学特性的研究奠定基础。
[Abstract]:In order to further understand the biological characteristics of a H6N6 subtype avian influenza virus (AIVA / WB / XJ / S1541 / 2015 H6N6) isolated from wild bird faeces in Xinjiang in 2015, the whole genome sequencing, genetic evolution analysis and infection test of BALB/c mice were carried out in this study. The results of sequence analysis showed that the amino acid sequence of HA protein cleavage site of the isolate was PQIETR (346A), with only one basic amino acid, which had the molecular characteristics of low pathogenicity AIV. In addition, the surface genes of the isolate were highly homologous to the A/swine/Guangdong/K6/2010 of a H6N6 subtype swine influenza virus, while the internal genes were highly homologous to the H6N6 subtype AIV A / P / 161 / 2014 H6N6, indicating the diversity of the gene sources of the isolate. The results of infection test in mice showed that the virus could be replicated effectively in the lungs and turbinate of mice. The titer of the virus was 5.33 logs10) EIDS50 / mLL 4.50 log10EID50/m / L, which indicated that the virus had the potential risk of infection in mammals. The results of this study provide a basis for the continuous monitoring of H6 subtype AIV and the study of related biological characteristics.
【作者单位】：中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点实验室;
【基金】：国家重点研发计划项目2016YFD0500201
【分类号】：S852.65

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