肉鸡生产链中肠炎沙门氏菌耐药性分析及ERIC-PCR分型

发布时间：2018-06-05 09:51

  本文选题：肠炎沙门氏菌 + 肉鸡　； 参考：《食品科学》2016年03期

【摘要】：目的:了解肉鸡生产链中肠炎沙门氏菌耐药性情况以及各生产环节菌株间亲缘关系,为临床用药及菌株追踪溯源提供依据。方法:采用微量肉汤稀释法对171株肠炎沙门氏菌进行13种抗菌药物药敏实验;采用肠杆菌基因间重复共有序列-聚合酶链式反应(enterobacterial repetitive intergenic consensus-polymerase chain reaction,ERIC-PCR)法对33株不同生产环节耐药菌株进行分子分型;采用SPSS 20.0软件、Gel-Pro Analyer 4.0和NTSYS pc 2.1软件进行分析。结果:171株肠炎沙门氏菌对13种药物耐药情况不同,其中对氨苄西林耐药情况最严重,耐药率高达90.06%,对恩诺沙星、氧氟沙星最为敏感,耐药率均仅为5.26%;不同环节间耐药性差异极显著(P0.01);多重耐药率高达95.32%,共有26种耐药谱型。不同环节的33株肠炎沙门氏菌分为4种(Ⅰ~Ⅳ)基因型,遗传相似性在66%~100%,Ⅰ型为优势基因型;基因型相同的菌株耐药谱不一定相同,反之,耐药谱相同的菌株基因型不一定相同。结论:肉鸡生产链条中沙门氏菌对多种抗菌药物产生耐药性,且耐药谱种类繁多;沙门氏菌能够沿着生产链进行水平传播,肉鸡场环节菌株基因型相对复杂,菌株基因型与耐药表型之间无明显相关性。
[Abstract]:Objective: to investigate the drug resistance of Salmonella enteritidis in the production chain of broilers and the relationship between strains in each production link, and to provide evidence for clinical use of drugs and tracing the origin of the strains. Methods: a total of 171 strains of Salmonella enteritidis were tested for antimicrobial susceptibility by broth dilution method. Enterobacterial repetitive intergenic consensus-polymerase chain reactionation ERIC-PCR method was used to type 33 drug-resistant strains in different production links, and SPSS 20.0 software, Gel-Pro Analyer 4.0 and NTSYS PC 2.1 software were used to analyze them. Results among 171 strains of Salmonella enteritidis, the resistance to ampicillin was the most serious, the resistance rate was as high as 90.06, and was most sensitive to enrofloxacin and ofloxacin. The drug resistance rate was only 5.26, the difference of drug resistance between different links was extremely significant (P 0.01), the multidrug resistance rate was up to 95.32, there were 26 drug resistance spectrum types. 33 strains of Salmonella enteritidis in different links were divided into 4 genotypes (鈪，

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