初情期启动过程中MeCP2和GnRH在母羊下丘脑的分布定位

发布时间：2018-06-05 10:55

本文选题：初情期 + 下丘脑　；参考：《中国畜牧杂志》2017年12期

【摘要】：本实验旨在研究甲基化CpG结合蛋白(MeCP2)和促性腺激素释放激素(GnRH)初情期启动过程中在母羊下丘脑的分布及二者关系。选取初情期前、临近初情期和初情期阶段雌性小尾寒羊各3只,采集下丘脑,利用免疫荧光双标记技术研究MeCP2和GnRH在母羊下丘脑视前区(POA)和弓状核(ARC)中的分布。结果表明:MeCP2和GnRH在下丘脑ARC和POA均有分布,且呈现共表达模式,ARC中共表达阳性细胞数在初情期显著高于初情期前期和临近初情期(P0.05),POA中共表达阳性细胞数初情期显著低于初情期前期和临近初情期(P0.05)。结果提示,MeCP2可能通过调控下丘脑GnRH的表达而参与母羊初情期启动的调控。
[Abstract]:The aim of this study was to investigate the distribution of methylated CpG-binding protein (MeCP2) and gonadotropin releasing hormone (GnRH) in the hypothalamus of ewe during the initial estrus. The hypothalamus was collected from three female small tail Han sheep before, near and near estrus, and the distribution of MeCP2 and GnRH in preoptic area (POA) and arcuate nucleus (ARC) of hypothalamus were studied by immunofluorescence double labeling technique. The results showed that both of them were distributed in the hypothalamus ARC and POA. The number of co-expression positive cells of ARC in the early estrus was significantly higher than that in the early estrus and near the early estrus, and the number of P0.05 POA co-positive cells in the early estrus was significantly lower than that in the early estrus and near estrus. The results suggest that MeCP2 may be involved in the regulation of the initiation of ewe at the beginning of estrus by regulating the expression of GnRH in the hypothalamus.
【作者单位】：吉林农业大学动物科学技术学院;吉林省科技创新平台管理中心;锦州医科大学畜牧兽医学院;
【基金】：国家自然科学基金(31402073) 辽宁省科技厅自然科学基金(2014022050) 吉林省教育厅科学技术研究项目(JJKH20170319KJ)
【分类号】：S826

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