鸭坦布苏病毒对雏鸭免疫系统的影响

发布时间：2018-06-06 21:53

本文选题：鸭坦布苏病毒 + 雏鸭　；参考：《中国兽医学报》2017年02期

【摘要】：为研究鸭坦布苏病毒(DTMUV)对雏鸭免疫系统的影响,本试验对5日龄雏鸭静脉接种DTMUV,并于接种后不同时间取雏鸭脾脏、胸腺、法氏囊进行组织病理学、抗原和凋亡检测。结果显示,DTMUV感染雏鸭的脾脏、胸腺、法氏囊均严重受损。接种DTMUV后4d,脾脏淋巴细胞减少,胸腺可见严重细胞崩解和大面积坏死区域,法氏囊滤泡轻度萎缩;接种后6d免疫器官病变最为严重,其中胸腺静脉严重栓塞,淋巴细胞变性坏死,空泡化也更加严重;接种后8d免疫器官病变均有所减轻,至16d,免疫器官结构基本恢复正常。通过免疫组织化学方法在感染雏鸭的脾脏、胸腺、法氏囊中均检测到DTMUV抗原;细胞凋亡试验显示DTMUV能够显著引起脾淋巴细胞凋亡。综上所述,DTMUV可严重损伤雏鸭免疫器官,且这种损伤常发生于感染早期,并于感染后8d出现好转。
[Abstract]:In order to study the effect of duck Tanbu Su virus (DTMUV) on the immune system of ducklings, the 5 day old ducklings were vaccinated with DTMUV, and the spleen, thymus and bursa of the ducks were histopathologically examined, antigen and apoptosis were detected at different time after inoculation. The results showed that the spleen, thymus and bursa of DTMUV infected ducks were severely damaged. 4D after DTMUV was inoculated with 4D. The spleen lymphocytes decreased, the thymus showed serious cell disintegration and large area necrosis area. The follicle of the bursa of Fabricius was mild atrophy. After inoculation, the most serious 6D immune organs were in the thymus vein, in which the thymus vein was seriously embolized, the lymphocyte degeneration and necrosis, and the vacuolization were more serious, and the immune organs of 8D were relieved after inoculation, to 16d and immune organs. DTMUV antigen was detected in the spleen, thymus and bursa of ducks by immunohistochemical method. Apoptosis test showed that DTMUV could induce apoptosis of spleen lymphocyte significantly. In conclusion, DTMUV can seriously damage the immune organs of ducks, and this kind of injury often occurs in early infection and 8D after infection. There's a turn for the better.
【作者单位】：山东农业大学动物科技学院;
【基金】：国家自然科学基金资助项目(31472199,31272583) 国家水禽产业技术体系资助项目(CARS-43-10) 山东省自然科学基金资助项目(ZR2012CM002)
【分类号】：S858.32

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