慢性冷热应激对三河牛血液生化指标及相关基因表达的影响

发布时间：2018-06-08 01:05

本文选题：三河牛 + 冷热应激　；参考：《中国农业大学学报》2017年08期

【摘要】：为研究冷热应激对三河牛血液生化指标及相关基因表达量的影响,利用实时荧光定量PCR和ELISA方法检测冷热应激期和非应激期血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)、葡萄糖(GLU)和尿素氮(BUN)含量及淋巴细胞中HSP70家族基因(HSPA1A、HSPA1B、HSPA8)mRNA表达量。结果显示:1)冷热应激期血清中CK和LDH活性相比非应激期均升高,热应激期升高尤为显著(P0.05);热应激期GLU含量相比非应激期显著降低(P0.05);热应激期BUN含量略低于非应激期,差异不显著;冷应激期GLU和BUN含量相比非应激期均显著升高(P0.05)。2)冷热应激期淋巴细胞中3个HSP70家族基因mRNA表达量相比非应激期显著升高(P0.05)。结果表明,慢性冷热应激改变了血液中酶的活性、糖和蛋白质的代谢,从而导致奶牛的一些组织细胞的损伤和营养代谢的紊乱,并冷热应激时血液淋巴细胞中HSP70家族基因mRNA表达量的上升,可在一定程度上反映其对动物机体的保护水平。
[Abstract]:In order to study the effects of cold and heat stress on blood biochemical indexes and related gene expression in Sanhe cattle, The contents of creatine kinase (CK), lactate dehydrogenase (LDH), glucose gluconium (GLU) and urea nitrogen bun (bun) in serum during cold and heat stress and non-stress period were detected by real-time fluorescence quantitative PCR and Elisa. The expression of HSP70 family gene, HSPA1A, HSPA1B, HSPA8 mRNA in lymphocytes was detected. The results showed that the activities of CK and LDH in serum during cold and heat stress period were higher than those in non-stress period, especially in heat stress period, GLU content in heat stress period was significantly lower than that in non-stress period, bun content in heat stress period was slightly lower than that in non-stress period, the content of bun in heat stress period was a little lower than that in non-stress period, the content of GLU in heat stress period was significantly lower than that in non-stress period. The contents of GLU and bun in cold stress period were significantly higher than those in non-stress period (P 0.05N. 2) the mRNA expression of three HSP70 family genes in lymphocytes during cold and heat stress was significantly higher than that in non-stress period. The results showed that chronic cold and heat stress changed the activity of enzymes, the metabolism of sugar and protein in blood, resulting in the injury of some tissues and cells and the disorder of nutritional metabolism in dairy cows. The increase of HSP70 gene mRNA expression in blood lymphocytes under cold and heat stress can reflect the protective level of HSP70 gene on animal body to some extent.
【作者单位】：内蒙古农业大学动物科学学院;内蒙古农业大学兽医学院;
【基金】：国家科技支撑项目(2015BAD03B04-5) 国家自然科学基金资助项目(31160473)
【分类号】：S823

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