自噬对布鲁氏菌在巨噬细胞内增殖情况的影响

发布时间：2018-06-08 04:38

本文选题：自噬 + 布鲁氏菌　；参考：《中国预防兽医学报》2017年12期

【摘要】：为探究自噬作用对布鲁氏菌(Brucella)在巨噬细胞(Mφ)内增殖活力的影响,本研究将体外培养的Mφ细胞分组,分别采用自噬激活剂雷帕霉素(Ra)或抑制剂3-MA处理细胞,并在不同处理的细胞组中接种表达绿色荧光的重组猪种布鲁氏菌(rB.suis-GFP),在接种后0.5 h~24 h,一方面通过细胞中LC3-Ⅱ含量及Mφ内B.suis数量的测定,另一方面通过激光共聚焦显微镜观察LC3-Ⅱ与B.suis共定位情况。结果显示,感染B.suis后0.5 h,Mφ即可发生自噬作用,并且Ra+Mφ+B.suis组LC3-Ⅱ含量显著高于其它组,表明B.suis的感染能够促进Mφ内LC3-Ⅱ的形成,增强Mφ的自噬作用;另一方面,Ra+Mφ+B.suis组胞内B.suis数量显著高于3-MA+Mφ+B.suis组与Mφ+B.suis组,表明自噬作用能够促进B.suis在Mφ内的增殖;但随着胞内B.suis数量的增加,各组LC3-Ⅱ的水平却逐渐下降,表明B.suis感染Mφ数量达饱和后,Mφ虽然启动自噬作用杀灭了部分B.suis,但却不能完全抑制B.suis在Mφ内大量增殖。本研究为初步确定自噬在B.suis增殖过程中的作用提供了依据。
[Abstract]:In order to explore the effect of autophagy on the proliferation of Brucella in macrophage (M phi), the M Phi cells were grouped in vitro by using autophagy activator rapamycin (Ra) or inhibitor 3-MA to treat the cells, and the recombinant porcine Brucella expressing green fluorescence (rB.suis-) was inoculated in different treated cell groups. GFP), after 0.5 h~24 h after inoculation, on the one hand, the content of LC3- II in cells and the number of B.suis in M Phi were measured. On the other hand, the co localization of LC3- II and B.suis was observed by laser confocal microscopy. The results showed that the autophagy could occur after the infection of B.suis 0.5 h, M phi, and the content of Ra+M Phi group was significantly higher than that of the other groups. The infection of B.suis can promote the formation of LC3- II in M phi and enhance the autophagy of M phi; on the other hand, the number of B.suis in the cell of Ra+M [Phi] +B.suis is significantly higher than that of 3-MA+M [1] +B.suis group and M [[Phi] +B.suis group, indicating that the autophagy can promote the proliferation of B.suis. When the number of B.suis infection M Phi was saturated, M Phi could kill part of B.suis, but it could not completely inhibit the proliferation of B.suis in M phi. This study provides a basis for the preliminary determination of the role of autophagy in the proliferation of B.suis.
【作者单位】：山东省滨州畜牧兽医研究院山东省滨州畜禽蜂胶疫苗研究开发推广中心;山东绿都生物科技有限公司;
【基金】：山东省自然资金项目(ZR2013CQ005) 山东省现代农业产业技术体系创新团队项目(SDAIT-10-07)
【分类号】：S852.61

【相似文献】