丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和血清细胞因子含量的影响

发布时间：2018-06-08 06:51

本文选题：丁酸梭菌 + 断奶仔猪　；参考：《动物营养学报》2017年08期

【摘要】：本试验旨在研究饲粮中添加丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和血清细胞因子含量的影响。试验采用单因子设计,选择28日龄、体重相近、健康状况良好的"杜×长×大"断奶仔猪12头,按完全随机区组设计分为2个组,每组6个重复,单栏饲养。对照组饲喂玉米-豆粕型基础饲粮,试验组饲喂基础饲粮+5×10~5CFU/g丁酸梭菌。试验预试期3 d,正试期14 d。结果表明:与对照组相比,试验组仔猪平均日增重显著提高7.83%(P0.05)、料重比降低5.26%(P0.05);试验组仔猪空肠NOD样受体蛋白(NLRP)3(P0.05)、NLRP6(P0.05)、NLRP12(P0.01)、封闭蛋白1(claudin-1)(P0.01)和紧密连接蛋白2(ZO-2)(P0.05)的mRNA相对表达水平显著或极显著上调,回肠claudin-1和ZO-2的mRNA相对表达水平极显著上调(P0.01);试验组仔猪空肠和回肠中乳酸杆菌数量显著提高(P0.05),空肠中大肠杆菌数量降低2.49%(P0.05),回肠中大肠杆菌数量显著降低(P0.05);试验组仔猪血清白细胞介素(IL)-1β含量降低5.47%、IL-10含量提高25.43%(P0.05)。综上所述,饲粮中添加丁酸梭菌能提高仔猪小肠屏障功能,调节机体免疫和肠道菌群平衡,促进仔猪生长。
[Abstract]:The purpose of this study was to study the effects of Clostridium butyrate on growth performance, intestinal barrier function and serum cytokine content in weaned piglets. A single factor design was used to select 12 weaned piglets of 28 days old with similar body weight and good health. According to the complete randomized block design, 12 weaned piglets were divided into 2 groups, 6 repeated in each group and raised in a single column. The control group was fed with corn-soybean meal basal diet, while the experimental group was fed with 5 脳 10 ~ (5) CFU / g Clostridium butyrate. The pre-test period was 3 days and the experimental period was 14 days. The results showed that compared with the control group, the average daily gain of piglets in the experimental group increased significantly by 7.83% and the feed to weight ratio decreased by 5.26%, and the relative expression levels of the NOD-like receptor protein NLRRP05NLRP6P0.05NLRP6P0.05P0.05NLRP12P0.01P0.01and close junction protein 2ZO-2P0.05) in the jejunum of the experimental group were significantly increased or extremely significantly increased compared with the control group, and the relative expression levels of NLRP6P0.05NLRP12P0.05NLRP12P0.01P0.01and P0.05) were significantly higher in the experimental group than those in the control group. The relative expression level of claudin-1 and ZO-2 in the ileum was significantly up-regulated, the number of Lactobacillus in jejunum and ileum in the experimental group was significantly increased, the number of Escherichia coli in jejunum was decreased by 2.49P0.05, the number of Escherichia coli in ileum was significantly decreased in test group, and the number of Escherichia coli in ileum was significantly decreased in test group. The content of IL-10 in serum of piglets decreased by 5.47% and increased by 25.43% (P0.05). To sum up, the addition of Clostridium butyrate in diet can improve the intestinal barrier function, regulate the immune system and the balance of intestinal flora, and promote the growth of piglets.
【作者单位】：天津市畜牧兽医研究所;天津市畜禽健康养殖技术工程中心;
【基金】：大北农杨胜先生门生社群项目(B2016008)
【分类号】：S828.5

【参考文献】