猪高糖高脂饲喂条件下肝脏转录组分析

发布时间：2018-06-10 07:21

本文选题：巴马香猪 + 转录组　；参考：《广西大学》2016年硕士论文

【摘要】：随着社会的发展,人们对猪肉品质的要求也在不断提高,如何提高肉的品质已成为现在畜牧业的研究重点。肌内脂肪的含量是评定肉品质的一个重要指标,加深对其的研究是相当必要的。脂肪代谢与肌内脂肪的沉积之间有着密切的关系,而肝脏脂肪代谢作为脂肪代谢中重要的一部分,也是影响肌内脂肪的沉积的一个重要因素。所以加深对肝脏脂肪代谢的研究有助于找到其对肌内脂肪沉积影响的分子机制。本研究中,首先在实验组巴马香猪的日粮中添加10%的糖和15%的脂肪,构建出肥胖猪模型。实验组和对照组在pH值、肉色、系水力和剪切力等肉质性状上都存在明显差异。血液生理指标中与血脂相关的指标都有明显的改变,这些指标的变化都与脂肪性状的改变有着密切的关系。然后将实验组和对照组的肝脏样品进行转录组测序,得到了613个在实验组和对照组肝脏中差异表达的基因。对这613个差异基因进行分析发现,有MOGAT2和NKX2-3等与脂肪类物质吸收相关的基因,也有SCD和ELOVL家族等与调控脂肪酸代谢相关的基因。对差异基因进行GO富集,发现这些差异基因主要富集于单体代谢过程、氧化还原过程、脂质代谢过程等,KEGG将这些差异基因富集于脂肪酸代谢信号通路、脂肪酸延长信号通路、PPAR信号通路和甘油脂代谢信号通路等与脂肪代谢相关的信号通路中。在转录组测序中还发现了在实验组和对照组出现了大量的可变剪切、SNPs和InDel差异,这些现象的发生,特别是可变剪切的差异可能直接造成了实验组和对照组编码蛋白质的不同,进而影响着代谢过程,但是这些现象是否对实验组和对照组的生理性状造成影响还有待更加深入的研究。本研究旨在利用转录组测序的方法,推测出影响肝脏脂肪代谢的分子生物学机理,从肝脏脂肪代谢的角度开展肉质品质的相关研究,为改善猪肉品质提供新的途径。
[Abstract]:With the development of society, people's demand for pork quality is also increasing, how to improve meat quality has become the focus of animal husbandry research. The content of intramuscular fat is an important index to evaluate meat quality. There is a close relationship between fat metabolism and intramuscular fat deposition. As an important part of fat metabolism, hepatic fat metabolism is also an important factor affecting intramuscular fat deposition. Therefore, further study of liver fat metabolism will help to find out the molecular mechanism of its effect on intramuscular fat deposition. In this study, the fat pig model was established by adding 10% sugar and 15% fat into the diet of the experimental group Bama Xiang pig. There were significant differences between the experimental group and the control group in pH value, meat color, water power and shear stress. There were obvious changes in blood physiological indexes related to blood lipids, and the changes of these indexes were closely related to the changes of fat traits. Then 613 differentially expressed genes were obtained from the liver samples of the experimental group and the control group. The 613 differentially expressed genes included MOGAT2, NKX2-3 and SCD and ELOVL family genes related to fatty acid metabolism. It was found that these differentially expressed genes were mainly enriched in monomer metabolism, redox process, lipid metabolism process and so on. KEGG enriched these differentially expressed genes in fatty acid metabolic signaling pathway. Fatty acid prolongation signaling pathway PPAR signaling pathway and glycerol metabolism signaling pathway related to fat metabolism. In transcriptome sequencing, a large number of variable-shear SNPs and InDel differences were found in the experimental and control groups. These phenomena, especially the variable-shear differences, may directly result in the difference of the encoded proteins between the experimental group and the control group. But whether the effects of these phenomena on the physiological characteristics of the experimental and control groups need to be further studied. The aim of this study was to speculate the molecular biological mechanism of liver fat metabolism by transcriptome sequencing, and to carry out the related research on meat quality from the point of view of liver fat metabolism, so as to provide a new way to improve pork quality.
【学位授予单位】：广西大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S828

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