不同来源油脂及剂量对仔猪生长性能和血清脂质指标以及肝脏胆固醇代谢的影响

发布时间：2018-06-11 14:54

本文选题：油脂 + 生长性能　；参考：《南京农业大学学报》2017年04期

【摘要】：[目的]为仔猪生产筛选适宜添加的油脂和剂量。[方法]将20窝初生日龄和初始体质量相近的7日龄长大二元杂交仔猪,随机分为基础日粮(CK)和4个处理组(T1~T4),每组4窝,每窝不少于8头。试验分为哺乳期(7~21 d)和断奶期(22~35 d)2个阶段,其中CK组均饲喂基础日粮,T1组在基础日粮上分别添加质量分数为0.16%和4%乳化椰子粉,T2组在基础日粮上分别添加0.32%和8%乳化椰子粉,T3组在基础日粮上分别添加0.16%乳化椰子粉和4%豆油,T4组饲喂基础日粮和在其基础上添加4%豆油。试验过程中,测定仔猪生长性能和血清脂质指标,并检测肝脏乙酰辅酶A乙酰基转移酶(ACAT)、3-羟-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)和胆固醇7α-羟化酶(CYP7A1)的活性变化,分析HMGCR、CYP7A1和低密度脂蛋白受体(LDLR)基因(mRNA)相对表达量变化。[结果]与对照相比,各处理对2个阶段仔猪生长性能均无显著影响(P0.05),添加不同剂量乳化椰子粉对哺乳阶段仔猪血清脂质指标无显著影响(P0.05),T2组能显著提高断奶阶段仔猪血清总胆固醇含量(P0.05)。同时,T2较T1组断奶仔猪血清总胆固醇含量显著提高(P0.05)。与对照相比,各处理HMGCR、CYP7A1和ACAT酶活性和CYP7A1、LDLR、HMGCR基因表达量无显著改变(P0.05),但T2组有增加HMGCR酶活性和HMGCR基因表达量趋势(P0.1)。[结论]添加不同剂量和类型油脂对试验仔猪生长性能无显著影响,但高剂量的乳化椰子粉能显著提高血清总胆固醇含量,并有增加肝脏合成胆固醇关键酶HMGCR酶活性和HMGCR基因表达的趋势。
[Abstract]:[objective] to screen suitable fat and dosage for piglet production. [methods] Twenty broilers of 7 day-old crossbred piglets of similar birth day and initial body mass were randomly divided into two groups: basal diet (CK) and four treatment groups (T _ (1) T _ (1) T _ (4), each group (n = 4) and not less than 8 (n = 8) per litter. The experiment was divided into two stages: the lactation period was 7 ~ 21 days) and the weaning period was 22 ~ 35 days. All the CK groups were fed basal diet with 0.16% and 4% emulsified coconut powder T2 respectively. The basal diet supplemented with 0.32% and 8% emulsified coconut powder T3 added 0.16% milk to the basal diet, respectively. Coconut powder and 4% soybean oil T4 group were fed with basal diet and 4% soybean oil. During the experiment, the growth performance and serum lipid index of piglets were measured, and the activities of liver acetyl coenzyme A acetyltransferase AcetylCoA A reductase (HMGCRs) and cholesterol 7 伪 -hydroxylase (CYP7A1) were measured. The relative expression levels of HMGCR-CYP7A1 and LDLRR gene were analyzed. [results] compared with the control, All treatments had no significant effect on the growth performance of piglets in two stages. The serum lipid index of piglets in the lactating stage was not significantly affected by adding different doses of emulsified coconut powder. The serum total cholesterol content of piglets at weaning stage was significantly increased in P0.05 / T _ 2 group. At the same time, the serum total cholesterol content of T 1 weaned piglets was significantly higher than that of T 1 weaning piglets. Compared with the control, the activity of CYP7A1 and ACAT and the expression of CYP7A1LDLRnHMGCR gene in HMGCR-CYP7A1 and ACAT and the expression of HMGCR gene were not significantly changed in all treatments, but the activity of HMGCR and the expression of HMGCR gene were increased in T2 group. [conclusion] there was no significant effect on growth performance of piglets supplemented with different doses and types of oils, but high dose of emulsified coconut powder could significantly increase serum total cholesterol content. There was also a tendency to increase the activity of HMGCR and the expression of HMGCR gene.
【作者单位】：南京农业大学养猪研究所;南京农业大学淮安研究院;江苏省如皋市长寿集团友联畜牧有限公司;
【基金】：国家科技支撑计划项目(2015BAD03B02-4) 中央高校基本科研业务费专项资金(KYCYL201502) 江苏省农业三新工程项目(SXGC[2015]319,SXGC[2016]275,SXGC[2016]146)
【分类号】：S828.5

【参考文献】