门静脉血氨对猪肝尿素循环和糖异生的影响

发布时间：2018-06-13 15:54

本文选题：门静脉血氨 + 肝　；参考：《畜牧兽医学报》2017年01期

【摘要】：旨在探索门静脉血氨对猪肝尿素循环和糖异生的影响。试验选取体重约20kg的杜长大仔猪8头,随机分成2组,每组4头,安装门静脉-肝静脉插管,分别灌注65和35mmol·L-1的氯化铵(NH4Cl),采集肝静脉血液,利用气相色谱-质谱联用技术分析血清代谢产物,同时测定肝组织尿素循环和糖异生相关酶基因表达及活性。结果表明,门静脉灌注高浓度的NH4Cl显著提高了血液中尿素、葡萄糖和葡萄糖6磷酸的含量(P0.05),显著降低了血液中丙氨酸、谷氨酸、天冬氨酸的含量(P0.05);高浓度的NH4Cl还显著上调了氨甲酰磷酸合成酶1(CPS1)、鸟氨酸转氨甲酰磷酸酶(OTC)、精氨酸酶1(Arg1)、磷酸烯醇式丙酮酸羧激酶(PCK)和葡萄糖6磷酸酶(G6PC)的基因表达(P0.05),同时相应酶活性分别提高了50.77%、44.47%、41.24%、46.99%、54.57%(P0.05)。结果提示,随着肝氨负荷的增加,尿素循环加强,诱导丙氨酸、天冬氨酸和谷氨酸等氨基酸代谢为尿素提供额外的氮源,生成的碳骨架用于肝糖异生。
[Abstract]:The purpose of this study was to explore the effect of portal vein blood ammonia on hepatic urea circulation and glucose allogenesis in pigs. Eight Duroc piglets of about 20kg weight were randomly divided into two groups, each group (n = 4). Portal venous catheterization was installed, and 65 and 35mmol L-1 ammonium chloride NH _ 4Cl _ 4 were infused respectively to collect hepatic vein blood. Serum metabolites were analyzed by gas chromatography-mass spectrometry (GC-MS). The expression and activity of urea-cycle and glycose-related enzymes in liver tissue were also determined. The results showed that the high concentration of NH4Cl in portal vein significantly increased the contents of urea, glucose and glucose-6-phosphoric acid in blood and decreased the levels of alanine and glutamate in blood. The content of aspartic acid was P0.05C, and the high concentration of NH4Cl also significantly up-regulated the gene expression of carbamyl phosphate synthase 1 (CPS1), ornithine transcarbamyl phosphatase (OTC1), arginase 1 (Arg 1), phosphoenolpyruvate carboxykinase (PCK) and glucose 6 phosphatase (G6PC). At the same time, the corresponding enzyme activities were increased by 50.77 and 44.47and 41.24, respectively, and 46.99 and 54.57, respectively. The results suggested that with the increase of liver ammonia load, the urea cycle increased, and the amino acid metabolism such as alanine, aspartic acid and glutamic acid were induced to provide additional nitrogen sources for urea, and the resulting carbon skeleton was used for liver glucose allogenesis.
【作者单位】：华中农业大学动物科技学院;
【基金】：国家重点基础研究计划(2013CB127304) 国家自然科学基金面上项目(31572409) "十三五"国家重点研发计划(2016YFD0500506)
【分类号】：S828

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