铅和镉对牦牛肾脏细胞的影响

发布时间：2018-06-14 09:55

本文选题：金属硫蛋白 + 牦牛肾脏细胞　；参考：《食品工业科技》2016年23期

【摘要】：为了揭示牦牛肾脏细胞在重金属胁迫下的毒理性影响,在培养出的肾脏细胞中加入不同浓度的重金属离子铅(Pb~2+)和镉(Cd~2+),提取总RNA,在逆转录酶的作用下生成c DNA,利用实时荧光定量技术检测牦牛肾脏细胞中金属硫蛋白mRNA的相对表达量。结果显示,将牦牛肾脏细胞中加入重金属浓度为0μmol/L的值设定为对照组,2~-ΔΔCt值设为1时,加入重金属铅的不同浓度0、5、10、20、30μmol/L的样品相对定量五种不同浓度的样品的2~-ΔΔCt分别为0.489、0.603、0.796、3.031;添加镉的五种不同浓度的样品的2~-ΔΔCt分别为5.280、3.530、8.110、11.630。通过差异性显著分析得出当重金属离子铅(Pb~2+)和镉(Cd~2+)添加10μmol/L时对肾脏细胞的生长有明显的抑制作用,相应的金属硫蛋白mRNA表达量较高,通过mRNA相对表达量的比较得出重金属镉的毒性比铅强,当重金属的添加量比较低时,重金属离子通过结合MT降低对肾脏细胞的毒害作用。
[Abstract]:In order to reveal the toxic effect of yak kidney cells under heavy metal stress, Different concentrations of heavy metal ions (Pb ~ (2 +) Pb2) and CD ~ (2 +) ~ (2 +) were added to the cultured renal cells to extract total RNAs and to produce c DNA by reverse transcriptase. The metallothionein in yak kidney cells was detected by real-time fluorescence quantitative technique. Relative expression of mRNA. The results showed that the concentration of heavy metal was 0 渭 mol / L in the kidney cells of yak, and the value of 螖 Ct was set to 1 when the concentration of heavy metal was 0 渭 mol / L in the control group. The relative quantification of the samples with different concentrations of heavy metal lead was 0.489U 0.603N 0.796N 3.031for the samples with different concentrations of cadmium, and 5.2803.5308.110ct for the samples with five different concentrations of cadmium, respectively, and 11.630for the samples with five different concentrations of cadmium. The difference analysis showed that 10 渭 mol / L of heavy metal ions Pb2) and CD ~ (2 +) could significantly inhibit the growth of renal cells, and the corresponding mRNA expression of metallothionein was higher than that of other heavy metal ions (Pb ~ (2 +) and CD ~ (2 +). The toxicity of cadmium was stronger than that of lead. When the addition of heavy metal was low, the toxicity of heavy metal ions to renal cells was reduced by binding MT.
【作者单位】：青海大学农牧学院动物科学系;
【分类号】：TS251.7

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