蝉花虫草粗多糖对鸡新城疫抗体水平影响的研究

发布时间：2018-06-14 10:45

本文选题：蝉花虫草粗多糖 + 鸡　；参考：《黑龙江畜牧兽医》2017年15期

【摘要】：为了研究蝉花虫草粗多糖的免疫调节作用,试验分为新城疫弱毒苗和灭活苗免疫两部分。新城疫弱毒苗试验选择1日龄雏鸡300只,随机分为Ⅰ组(对照组)、蝉花虫草粗多糖低(Ⅱ)、中(Ⅲ)、高(Ⅳ)剂量组、Ⅴ组(黄芪组)。7~28日龄雏鸡灌服多糖或生理盐水,14,28日龄滴鼻点眼新城疫Ⅳ系弱毒疫苗,分离14,21,28,35,42,49,56日龄鸡血清并检测新城疫抗体效价。灭活苗试验选择53日龄鸡80只,分为Ⅰ组、Ⅱ组、Ⅲ组、Ⅳ组,在63~65日龄时灌服多糖或生理盐水,63日龄时免疫新城疫油乳剂灭活疫苗2羽份,分离70,77,84日龄鸡血清并检测新城疫抗体效价。结果表明:在新城疫弱毒苗试验中,7~14日龄雏鸡添加100 mg/kg蝉花虫草粗多糖能有效延长新城疫母源抗体的保护期,提升成活率,且与弱毒苗作用后有效保护鸡群新城疫抗体水平维持在较高水平。在灭活苗试验中,对于60日龄鸡群,添加50 mg/kg蝉花虫草粗多糖可以起到明显提高新城疫抗体水平的效果。说明添加一定剂量的蝉花虫草粗多糖能有效提升鸡新城疫疫苗的免疫效果。
[Abstract]:In order to study the immunomodulatory effect of the crude polysaccharide of Cordyceps cicadas, the experiment was divided into two parts of the vaccinia and inactivated vaccine, which were divided into two 1 days old chicks, which were randomly divided into group I (control group), low polysaccharide (II) of Cordyceps cicadas (II), middle (III), high (IV) dose group, and group V (Huang Qizu).7~28 day old chicks were filled with polysaccharide or Physiological saline, 14,28 day old Newcastle disease IV weak virus vaccine, isolated 14,21,28,35,42,49,56 day old chicken serum and detecting the antibody titer of Newcastle disease. The inactivated vaccine test selected 80 53 day old chickens, divided into group I, group II, group III and group IV, filled with polysaccharide or physiological saline at 63~65 day age, and immunized Newcastle disease oil emulsion inactivated at 63 days of age. The antibody titer of Newcastle disease (NDV) was isolated from the 2 feathers of 70,77,84 day old chicken and the antibody titer of Newcastle disease was detected. The results showed that in the test of Newcastle disease and weak virus, the addition of 100 mg/kg of cicadeca cicada's crude polysaccharide could effectively prolong the protection period of NDV mother antibody, improve the survival rate, and effectively protect the level of the antibody level of Newcastle disease after the action of the weak virus vaccine. In the test of inactivated vaccine, the effect of adding 50 mg/kg cicada Cordyceps polysaccharide to the 60 day old chicken group could obviously improve the antibody level of Newcastle disease. It is indicated that the addition of a certain dose of cicadeca cicadas polysaccharide can effectively improve the immune effect of the Newcastle disease vaccine.
【作者单位】：温州科技职业学院;浙江省农业科学院畜牧兽医研究所;浙江省亚热带作物研究所;
【基金】：浙江省重大优先项目(2008C12044-3) 2015年浙江省新苗人才计划项目(2015R458001) 浙江省教育厅高等学校访问工程师校企合作项目(FG2014197)
【分类号】：S858.31

【相似文献】