利用MiSeq测序技术分析锦江牛瘤胃细菌多样性

发布时间：2018-06-14 18:48

  本文选题：锦江牛 + 瘤胃细菌　； 参考：《动物营养学报》2016年01期

【摘要】：本研究旨在利用MiSeq高通量测序技术揭示锦江牛瘤胃细菌组成及多样性。选用3头装有永久性瘤胃瘘管的锦江牛成年公牛[体重(400±20)kg],采集瘤胃液样品后提取细菌DNA,对瘤胃细菌的16S rDNA序列V4~V5区进行MiSeq测序,分析物种的丰度、分布及α-多样性。结果表明:3个样本共获得56 763条高质量16S rDNA序列,946个分类操作单元(OTU),锦江牛瘤胃细菌丰富度指数Chao指数、Ace指数分别为836、841,α-多样性指数Shannon指数、Simpson指数分别为4.96、0.021 5;经过物种注释,15个门、23个纲、26个科以及44个属在所有样品中被鉴定。在门水平,拟杆菌门(Bacteroidetes)(64.37%)、厚壁菌门(Firmicutes)(21.20%)和变形菌门(Proteobacteria)(7.59%)数量最多;在纲水平,主要包括拟杆菌纲(Bacteroidia)(52.88%)和梭菌纲(Clostridia)(17.03%);在科水平,主要包括普雷沃氏菌科(Prevotellaceae)(36.26%)、瘤胃菌科(Ruminococcaceae)(9.33%)和毛螺菌科(Lachnospiraceae)(4.89%);在属水平,优势细菌主要包括普雷沃氏菌属(Prevotella)(28.97%)、帕拉普氏菌属(Paraprevotella)(3.08%)、理研菌属(Rikenella)(2.28%)、梭菌_Ⅳ(ClostridiumⅣ)(1.77%)、解琥珀酸菌属(Succiniclastium)(1.62%)和瘤胃球菌属(Ruminococcus)(1.53%)。结果证明,锦江牛瘤胃细菌多样性相对较低;锦江牛瘤胃中最优势菌门是拟杆菌门,其次是厚壁菌门;普雷沃氏菌属是锦江牛瘤胃中最优势细菌属。
[Abstract]:The aim of this study was to reveal the composition and diversity of rumen bacteria in Jinjiang cattle by using MiSeq high throughput sequencing technique. Three adult bulls with permanent rumen fistula [body weight: 400 卤20)kg] were collected from rumen fluid to extract bacterial DNA. The 16s rDNA sequence of rumen bacteria was sequenced in V4 / V5 region. The species abundance, distribution and 伪 -diversity were analyzed. The results showed that a total of 56,763 high quality 16s rDNA sequences were obtained from three samples. The results showed that 946 taxonomic operating units were obtained from the three samples. The rumen bacterial richness index (Chao) and 伪 -diversity index (Shannon index and Simpson index) of Jinjiang cattle were 836 841 and 4.96 0. 021 5, respectively. Species notes, 15 phylum, 23 classes, 26 families and 44 genera were identified in all samples. 鍦ㄩ棬姘村钩,鎷熸潌鑿岄棬(Bacteroidetes)(64.37%),鍘氬�佽弻闂�(Firmicutes)(21.20%)鍜屽彉褰㈣弻闂，

本文编号：2018597