过瘤胃胆碱对黔北麻羊瘤胃发酵及血浆生化指标的影响

发布时间：2018-06-14 20:21

本文选题：过瘤胃胆碱 + 瘤胃发酵　；参考：《动物营养学报》2017年01期

【摘要】：本试验旨在研究过瘤胃胆碱(RPC)对成年黔北麻羊瘤胃发酵及血浆生化指标的影响。以体况良好,年龄、体重[(35.0±1.2)kg]一致的成年黔北麻羊6只为试验动物,分为对照组和试验组,每组3个重复,每个重复1只羊。采用交叉试验设计,对照组饲喂基础饲粮,不含RPC;试验组补饲10 g/d的RPC。试验持续30 d,分2期,每期15 d;每期最后1 d采集瘤胃液测定p H、氨态氮浓度、微生物蛋白产量、挥发性脂肪酸(乙酸、丙酸、丁酸)浓度、纤维素酶(微晶纤维素酶、羧甲基纤维素酶、纤维二糖酶、木聚糖酶)活性,同时采血测定血浆葡萄糖、尿素氮、总蛋白、白蛋白、总胆固醇、甘油三酯、肌酐浓度。结果显示:1)RPC对山羊采食速度没有影响,对采食量没有显著影响(P0.05);2)RPC对瘤胃液p H、氨态氮浓度、微生物蛋白产量、挥发性脂肪酸浓度及4种纤维素酶活性影响较小,2组间差异均不显著(P0.05);3)RPC对血浆生化指标影响较小,2组间差异均不显著(P0.05)。由此可见,补饲10 g/d的RPC对成年黔北麻羊的采食量、瘤胃发酵参数和血浆生化指标没有显著影响。
[Abstract]:The purpose of this study was to study the effects of rumen choline (RPC) on rumen fermentation and plasma biochemical indexes in adult Qianbei Ma sheep. Six adult Qianbei Ma sheep with good physical condition, age and body weight [35. 0 卤1.2)kg] were divided into two groups: control group and experimental group, with 3 replicates in each group and 1 sheep in each group. The control group was fed with basic diet without RPC, and the experimental group was supplemented with 10 g / d RPCs. The experiment lasted for 30 days and was divided into 2 phases for 15 days; rumen fluid was collected on the last day of each phase for determination of pH, ammonia nitrogen concentration, microbial protein production, volatile fatty acid (acetic acid, propionic acid, butyric acid) concentration, cellulase (microcrystalline cellulase). Carboxymethyl cellulase, cellulase and xylanase were used to determine plasma glucose, urea nitrogen, total protein, albumin, total cholesterol, triglyceride and creatinine concentration. The results showed that 1% RPC had no effect on feed intake rate of goats, but had no significant effect on feed intake. There was no significant difference in the concentration of volatile fatty acids and the activity of four kinds of cellulase between the two groups. It can be seen that supplementary feeding of 10 g / d RPC has no significant effect on feed intake, rumen fermentation parameters and plasma biochemical indexes of adult Qianbei Ma sheep.
【作者单位】：贵州大学动物科学学院;贵州大学新农村发展研究院;贵州省草业研究所;黔东南民族职业技术学院;
【基金】：国家自然科学基金(31360563) 贵州山区牧草产业化生产技术研究集成与应用(黔科合重大专项字[2014]6017号) 贵州大学科技兴村人才培育基地建设项目黔东南农作物秸秆加工利用营养价值评定(黔东南科合J字[2016]015号)
【分类号】：S827.5

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