玉米赤霉烯酮降解菌的筛选及其活性检测

发布时间：2018-06-15 03:20

  本文选题：真菌毒素 + 玉米赤霉烯酮　； 参考：《畜牧兽医学报》2017年04期

【摘要】：玉米赤霉烯酮(Zearalenone,ZEN)是危害动物健康的重要真菌毒素之一,笔者旨在筛选出能够有效降解ZEN的微生物,并对其降解特性以及降解产物的致毒活性进行检测,以期筛选出高效降解ZEN的菌株。以ZEN为唯一碳源进行初筛,以菌株发酵液对ZEN的降解率为复筛指标,对降解率最高的菌株进行16SrRNA基因测序分析;并对筛选出的菌株对ZEN的降解特性进行研究,对其降解产物在MCF-7细胞上进行活性检测。初筛获得40株能生长良好的细菌,复筛筛选出降解率最高H6菌株,经16SrRNA基因测序分析,该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。在37℃、180r·min-1、72h时,该菌株发酵液对1μg·mL~(-1) ZEN的降解率为95.6%,其上清液和菌悬液的降解率分别为68.93%和28.70%,对20μg·mL~(-1) ZEN的降解率为85.8%;菌株上清液经蛋白酶K、蛋白酶K+SDS和热处理后,其降解活性明显降低;该菌株ZEN的降解产物在MCF-7细胞上的增殖率显著降低。结果表明,本研究筛选出能有效降解ZEN的菌株H6,经鉴定判断为解淀粉芽孢杆菌(B.amyloliquefaciens),该菌株分泌的胞外酶能够降解ZEN,使其类雌激素毒性显著降低。
[Abstract]:Zen is one of the most important fungal toxins harmful to animal health. The purpose of this study was to screen out the microorganisms that could effectively degrade Zen, and to detect the degradation characteristics and toxic activity of the degradation products. The aim of this study was to screen the strain that could degrade Zen efficiently. Zen was used as the sole carbon source, the degradation rate of Zen was used as the index of double screening, the 16s rRNA gene of the strain with the highest degradation rate was sequenced, and the degradation characteristics of Zen were studied. The biodegradation products were detected in MCF-7 cells. 40 strains of bacteria which could grow well were screened out, and the highest degradation rate of H6 strain was screened. The strain was Bacillus amyloliquefaciensus by 16s rRNA gene sequencing analysis. At 37 鈩，

本文编号：2020372