大豆抗原蛋白β-conglycinin线性表位及其不同种属动物过敏血清结合能力比较

发布时间：2018-06-15 06:14

本文选题：β-伴大豆球蛋白 + 表位　；参考：《东北农业大学学报》2017年01期

【摘要】：试验通过生物信息学方法预测β-conglycinin线性表位,综合已发表文献筛选出四段具有代表性氨基酸序列合成短肽,采用ELISA和点杂交法检测表位肽段与仔猪、犊牛、兔三种不同种属动物过敏血清结合程度差异。结果表明:通过生物信息学方法预测合成的肽段均为β-conglycinin表位肽;三种动物过敏血清与四段表位肽结合能力存在显著差异,其中四段表位肽与仔猪、犊牛过敏血清结合能力显著高于兔过敏血清(P0.05);同一动物与四段不同肽段结合敏感程度差异显著(P0.05),兔过敏血清与肽段β1结合能力最强,仔猪过敏血清与α1肽段结合能力最强,犊牛过敏血清与肽段β2肽段结合能力最强。结果为寻找β-conglycinin最佳优势表位提供理论支持,同时为揭示大豆抗原蛋白种属间致敏差异机理奠定基础。
[Abstract]:The bioinformatics method was used to predict 尾 -conglycinin linear epitopes. Four segments of representative amino acid sequences were selected to synthesize short peptides. Elisa and dot hybridization were used to detect epitope peptides, piglets and calves. The degree of allergic serum binding in three different species of rabbits was different. The results showed that the synthesized peptides were all 尾 -conglycinin epitope peptides predicted by bioinformatics, and the binding ability of three kinds of animal allergic sera to four epitope peptides was significantly different from that of piglets. The binding ability of allergy serum of calf was significantly higher than that of allergic serum of rabbit (P 0.05), the sensitivity of the same animal to four different peptides was significantly different (P 0.05), the binding ability of rabbit allergic serum to peptide 尾 1 was the strongest, and the ability of allergy serum of piglets to bind to 伪 1 peptide was the strongest. Calf allergy serum has the strongest binding ability to peptide 尾 2 peptide. The results provided theoretical support for searching for the best dominant epitope of 尾 -conglycinin, and laid a foundation for revealing the mechanism of sensitizing difference among soybean antigen protein species.
【作者单位】：动物生产及产品质量安全教育部重点实验室吉林省动物营养与饲料科学重点实验室吉林农业大学动物科学技术学院;
【基金】：国家自然科学基金(31572439,31572415) 吉林省自然科学基金(20160101348JC) 吉林省教育厅“十二五”科学技术研究项目(2015198)
【分类号】：S816

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