藏鸡FTO基因表达的时空特性及与肌内脂肪含量的相关分析

发布时间：2018-06-16 16:09

本文选题：藏鸡 + FTO基因　；参考：《畜牧兽医学报》2017年07期

【摘要】：旨在获得藏鸡FTO基因序列,并分析其生物学特性,阐明其组织及时序表达规律,同时揭示该基因在不同发育阶段肌肉组织中的表达水平与肌内脂肪含量的关系。选取1~210日龄健康藏鸡为试验材料,采集心、肝、脾、肺、肾、胸肌、腿肌和皮下脂肪组织样品,提取组织总RNA,利用RT-PCR方法克隆FTO基因序列,同时利用实时荧光定量PCR检测其在各组织及不同发育阶段的mRNA表达丰度,并将其表达量与肌内脂肪含量进行相关分析。结果表明,获得藏鸡FTO的基因序列长1 585bp(GenBank登陆号:KY366175),其中CDS为1 524bp,5′UTR 35bp和3′UTR 26bp,编码507个氨基酸,具有一个N端结构域和一个C端结构域。藏鸡与原鸡FTO氨基酸相比发生了6个氨基酸突变。FTO基因在藏鸡的各个组织中广泛表达,在脾组织中的表达水平极显著高于其他组织(P0.01),在脂肪组织和肺组织也存在较高水平的表达。时序表达谱显示,FTO基因在藏鸡胸肌和腿肌具有不同的表达模式,在胸肌中的表达水平随生长日龄的增加呈逐渐下降的趋势,而在腿肌中的表达水平随生长日龄的增加呈先上升后下降的趋势。藏鸡在不同发育阶段,不同肌肉组织中FTO基因表达水平与IMF含量具有不同程度的相关性,其中FTO基因表达水平与藏鸡胸肌IMF含量呈负相关,并且在母鸡中的相关性达到显著水平(P0.05)。而公鸡FTO基因表达水平与腿肌IMF含量呈正相关(在119~210日龄阶段r=0.601,P0.05),母鸡则相反。研究结果提示,FTO基因可能在藏鸡生长发育过程中对肌内脂肪沉积起重要调控作用,本试验结果将为藏鸡FTO基因的结构和功能的进一步研究奠定基础。
[Abstract]:The aim of this study was to obtain the FTO gene sequence of Tibetan chicken, to analyze its biological characteristics, to elucidate its tissue and temporal expression patterns, and to reveal the relationship between the expression level of FTO gene and muscle fat content in different developmental stages. The samples of heart, liver, spleen, lung, kidney, pectoralis muscle, leg muscle and subcutaneous adipose tissue were collected and the FTO gene sequence was cloned by RT-PCR. At the same time, real-time fluorescent quantitative PCR was used to detect the mRNA expression abundance in various tissues and different developmental stages, and the correlation between the expression level and the intramuscular fat content was analyzed. The results showed that the gene sequence of Tibetan chicken FTO was 1 585bpN GenBank accession number: KY366175, in which CDS was 1 524 BP 35bp and 3Amino 3 UTR 26bp, encoding 507 amino acids, with a N-terminal domain and a C-terminal domain. There were 6 amino acid mutations. FTO gene was widely expressed in different tissues of Tibetan chicken compared with the original chicken FTO amino acid. The expression level in spleen tissue was significantly higher than that in other tissues, and there was also a high expression level in adipose tissue and lung tissue. The expression patterns of FTO gene in pectoralis muscle and leg muscle of Tibetan chicken were different, and the expression level of FTO gene in pectoralis muscle decreased gradually with the increase of growth age. However, the expression level in leg muscle increased first and then decreased with the increase of growth age. The expression level of FTO gene was correlated with the content of IMF in different muscle tissues, and the expression level of FTO gene was negatively correlated with the content of IMF in pectoralis muscle of Tibetan chicken. The correlation among hens was significant (P 0.05). The expression level of FTO gene was positively correlated with the content of IMF in leg muscle of male chickens (0.601g / kg P0.05A) at the age of 110 ~ 210d, but the contrary was observed in hens. The results suggest that FTO gene may play an important role in the regulation of intramuscular fat deposition during the growth and development of Tibetan chickens. The results of this study will lay a foundation for further study on the structure and function of FTO gene in Tibetan chickens.
【作者单位】：西南民族大学生命科学与技术学院;青藏高原动物遗传资源保护与利用四川省重点实验室;
【基金】：四川省科技支撑计划“十三五”育种攻关项目(2016NYZ0043)
【分类号】：S831.2

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