6-苄氨基嘌呤对大鼠小肠缺血再灌注损伤的影响

发布时间：2018-06-17 01:39

  本文选题：-苄氨基嘌呤 + 缺血再灌注　； 参考：《动物营养学报》2017年09期

【摘要】：本试验旨在研究6-苄氨基嘌呤(6-BA)对大鼠小肠缺血再灌注(I/R)损伤的保护作用。选取80只雄性SD大鼠,随机分为4组,即对照组、I/R模型组和低、高剂量6-BA组。低、高剂量6-BA组于术前3周分别连续灌胃10、20mg/kg的6-BA,对照组和I/R模型组灌胃同体积的生理盐水,每天1次。对照组在暴露肠系膜上动脉后不做阻断;I/R模型组和低、高剂量6-BA组阻断肠系膜血管30min后再灌注60min。随后取大鼠空肠组织分别进行总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量检测;采用单细胞凝胶电泳法检测细胞DNA损伤程度,采用免疫组化法检测半胱氨酸天冬氨酸特异性蛋白酶3(Caspase-3)表达情况。结果显示,与I/R模型组相比,补充10、20 mg/kg的6-BA后,T-SOD、GSH-Px活性显著升高(P0.05),MDA含量显著降低(P0.05);小肠细胞DNA的拖尾现象好转,尾部DNA含量和拖尾率都显著低于I/R模型组(P0.05);Caspase-3阳性表达细胞数量显著减少(P0.05)。由此可见,10、20 mg/kg的6-BA能有效防护I/R对小肠的损伤,且20mg/kg的6-BA作用效果尤为明显。
[Abstract]:The aim of this study was to investigate the protective effect of 6-benzyl aminopurine 6-BA) on intestinal ischemia reperfusion injury in rats. Eighty male Sprague-Dawley rats were randomly divided into 4 groups: control group, I / R model group and low and high dose 6-BA group. The low and high dose 6-BA groups were intragastrically perfused with 10 ~ 20 mg / kg 6-BA for 3 weeks before operation. The control group and the I / R model group were given the same volume of normal saline once a day. The control group did not block the superior mesenteric artery after exposure to the superior mesenteric artery and the model group was low and low. The high dose 6-BA group blocked the mesenteric vessel 30min for 60 minutes after reperfusion. The total superoxide dismutase (T-SODX), glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content were detected in jejunum of rats, and DNA damage was detected by single cell gel electrophoresis. The expression of cysteine aspartate specific proteinase 3 (Caspase-3) was detected by immunohistochemical method. The results showed that compared with the I / R model group, the activity of GSH-Px of T-SODX was significantly increased after 6-BA supplementation for 10 ~ 20 mg/kg, and the MDA content of P0.05 was significantly decreased, and the tail trailing phenomenon of intestinal cells was improved. The tail DNA content and tail dragging rate were significantly lower than those in the I / R model group, and the number of Caspase-3 positive cells was significantly decreased in the I / R model group. It can be seen that 6-BA of 10 ~ 20 mg/kg can effectively protect the small intestine from injury caused by I / R, and the effect of 6-BA of 20mg/kg is especially obvious.
【作者单位】： 河南科技大学动物科技学院;
【基金】：国家自然科学基金(U1504325)
【分类号】：S816
，

本文编号：2029024