邻苯二甲酸二（2-乙基己基）酯致雌性鹌鹑生殖毒性研究

发布时间：2018-06-17 02:22

本文选题：邻苯二甲酸二(2-乙基己基)酯 + 卵巢　；参考：《东北农业大学》2017年硕士论文

【摘要】：邻苯二甲酸二(2-乙基己基)酯(DEHP)作为内分泌干扰物,广泛存在于环境中。由于DEHP可以通过多种途径进入机体,因此其对动物健康和生态环境有严重威胁。然而,DEHP对雌性生殖系统的影响尚未得到充分研究,对禽类雌性生殖毒性研究目前仍属于空白。本研究以雌性鹌鹑作为试验动物,通过灌胃染毒,观察鹌鹑整体状态和卵巢输卵管的病理变化,检测卵巢抗氧化功能、激素水平、下丘脑-垂体-卵巢轴性激素相关因子及相关激素受体的表达。结果表明:(1)DEHP暴露引发鹌鹑卵巢组织病理组织学和超微结构改变,扰乱性激素水平,诱发卵巢发育障碍。(2)DEHP暴露能够诱导鹌鹑卵巢组织中脂质过氧化物生成过量,抑制抗氧化酶系统活性,进而诱发氧化应激,发挥损伤鹌鹑卵巢组织的作用。(3)DEHP暴露能够影响孕激素受体(PGR)和雌激素受体(ERα与ERβ)等受体的表达,干扰下丘脑-垂体-卵巢轴类固醇激素相关因子的表达,阻断类固醇激素的合成。(4)DEHP暴露能干扰雌性鹌鹑下丘脑-垂体-卵巢性腺轴的正常调节功能,扰乱生殖内分泌系统稳态,这是其致鹌鹑雌性生殖毒性的重要机制之一。得出结论,DEHP暴露能够引起雌性鹌鹑卵巢组织的损伤,干扰下丘脑-垂体-卵巢性腺轴的调控功能,阻碍性激素的合成,使性激素分泌紊乱,从而对鹌鹑生殖系统产生严重的毒性作用,且该毒性存在剂量依赖效应。
[Abstract]:Diphenyl phthalate diethylhexyl) ester (DEHP), as an endocrine disruptor, is widely used in the environment. DEHP is a serious threat to animal health and ecological environment because it can enter the body through many ways. However, the effect of DEHP on female reproductive system has not been fully studied, and the study on female reproductive toxicity of poultry is still blank. In this study, female quails were used as experimental animals to observe the whole state of quails and the pathological changes of oviducts, and to detect the antioxidant function and hormone level of the ovaries. Expression of hypothalamus-pituitary-ovary axis hormone related factors and related hormone receptors. The results showed that DEHP exposure induced histopathological and ultrastructural changes in the ovary of quails, disturbed the level of sex hormones, and induced ovarian dysplasia. DEHP exposure could induce excessive production of lipid peroxides in quail ovarian tissues. Inhibiting the activity of antioxidant enzyme system, then inducing oxidative stress, which could affect the expression of progesterone receptor (PGR), estrogen receptor ER 伪 (ER 伪) and estrogen receptor (ER 尾) and so on. Interfering with the expression of steroid hormone related factors in hypothalamus-pituitary-ovary axis and blocking steroid hormone synthesis. (4) DEHP exposure can interfere the normal regulation function of hypothalamus-pituitary-ovarian gonad axis in female quails and disrupt the steady state of reproductive endocrine system. This is one of the important mechanisms of its reproductive toxicity to female quails. Conclusion DEHP exposure can damage the ovarian tissue of female quails, interfere with the regulation function of hypothalamus-pituitary-ovarian gonad axis, hinder the synthesis of sex hormones, and make sex hormone secretion disorder. Therefore, there was a serious toxic effect on the reproductive system of quails, and there was a dose-dependent effect on the toxicity.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.39

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