牛血红蛋白源抗菌肽P3及其类似肽的生物信息学分析

发布时间：2018-06-17 22:45

本文选题：牛血红蛋白 + 抗菌肽　；参考：《中国畜牧兽医》2017年01期

【摘要】：为研究牛血红蛋白源抗菌肽P3及其类似肽(JH-0、JH-1、JH-2、JH-3)的生物学功能,本研究利用在线软件分析了抗菌肽P3及其类似肽的生物信息学特性,发现这5个抗菌肽均带正电荷,等电点均大于8.00,均为疏水蛋白,定位于细胞外,不含有跨膜区、信号肽序列和糖基化位点,P3和JH-2的二级结构为α-螺旋+β-折叠,JH-3为100%的α-螺旋,JH-0和JH-1为无规则卷曲结构,P3、JH-2和JH-3在第13位氨基酸即苏氨酸(Thr,T)有一个糖基化位点。根据分析结果推测抗菌肽P3及其类似肽可作用于细胞壁或细胞膜,从而使菌体死亡。
[Abstract]:In order to study the biological function of bovine hemoglobin derived antimicrobial peptide P3 and its analogous peptide JH-0, the bioinformatics characteristics of antimicrobial peptide P3 and its analogous peptides were analyzed by on-line software. It was found that the five antimicrobial peptides were positively charged. The isoelectric points were more than 8.00, they were hydrophobic proteins, which were located outside the cells and did not contain the transmembrane region. The signal peptide sequence and glycosylation sites P3 and JH-2 have a glycosylation site at the 13th amino acid, namely, threonine thr (THR) with 100% 伪 -helix JH-0 and JH-1 as irregularly curled structure (P _ 3H _ 2 and JH _ 3), and the secondary structures of the signal peptide and glycosylation sites are 伪 -helix 尾 -foldout (JH-3) and JH-1 (100%). The results suggested that the antibacterial peptide P3 and its analogous peptides could act on the cell wall or cell membrane, resulting in the cell death.
【作者单位】：扬州大学兽医学院;河南省新乡市畜产品质量监测检验中心;河南科技学院动物科技学院;
【基金】：国家自然科技基金项目(31372469、31672559) 河南省高校科技创新团队支持计划(15IRTSTHN) 河南科技学院高层次人才启动项目(2014020)
【分类号】：S859.79

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