类固醇激素对昆明小鼠宫颈和宫体细胞脂滴形成的影响

发布时间：2018-06-19 03:50

本文选题：脂滴 + 小鼠　；参考：《山东农业大学》2015年硕士论文

【摘要】：子宫颈是雌性动物维持妊娠和进行分娩的重要器官,子宫颈的成熟对引产成功与否、分娩是否顺利以及产程的长短来说都至关重要。子宫颈成熟是受到许多细胞因子和激素的调节,是一个生化过程与炎症反应过程。子宫体也是雌性动物重要的生殖器官,它是孕育胎儿的场所,在精子迁移、受精卵着床以及胚胎、胎儿的生长发育及分娩过程中具有不可替代的作用。子宫颈中含有成纤维细胞、上皮细胞、内皮细胞以及前脂肪细胞和脂肪细胞。成纤维细胞来源于胚胎时期的中胚层,是结缔组织内的主要细胞之一。具有一定的分化能力,并参与创伤修复、诱导成骨和成脂肪过程,还在一定条件下与纤维细胞和间充质干细胞进行相互转化。脂肪组织还可作为内分泌器官参与集体的生理和病理过程。前脂肪细胞或脂肪细胞就能够分泌其中的细胞因子和激素,而本课题组就在分娩山羊的子宫颈中发现了含有大脂滴的脂肪细胞。在小鼠的子宫颈中也发现了含大脂滴的脂肪细胞的存在,并对小鼠子宫颈中脂滴的数量、分布以及变化等做了相应的研究,取得了一定的成果。脂滴最初被认为仅是用来储存能量的一种颗粒,这种颗粒类似于糖原,是一种“惰性”的细胞内物质,但随着脂滴蛋白质组学研究的不断深入,研究人员发现,脂滴还是一个复杂、动态变化、活动旺盛的多功能细胞器。它能沿着细胞的骨架运动,并能够与其它细胞器相互作用,可能在信号传导、膜转运、脂类存储与代谢等过程中扮演着非常重要的角色。脂滴在脂肪细胞、肝细胞、肌肉细胞以及肾上腺皮质细胞等多种组织细胞中广泛存在。本实验以昆明小白鼠为研究对象,对小鼠子宫颈组织中脂滴是否受激素的影响进行研究。采集成年小鼠子宫颈和经摘除卵巢注射激素(雌二醇、孕酮、地塞米松和米非司酮)处理的小鼠子宫颈,将子宫颈做冰冻切片并油红O染色;在洁净工作台内采集小鼠子宫颈,在含有胎牛血清的DMEM培养基中培养,并在培养基中加入一定浓度的激素,分别在无血清无激素、有血清无激素和有血清激含素浓度分别是10 nmol/L、20 nmol/L、40 nmol/L、80 nmol/L的四种激素的培养基中培养,油红O染色观察。结果显示:注射孕酮和地塞米松的小鼠的子宫颈中阳性率较高,注射米非司酮的阳性率较低;未摘卵巢未注射激素的小鼠的子宫体中阳性率最高,其他各组与子宫颈的结果基本一致。细胞培养时加孕酮的培养基培养的细胞中脂滴较加米非司酮的细胞中的脂滴含量高,经含地塞米松的培养基培养细胞中的脂滴的含量也高于在含米非司酮的培养基中培养的细胞脂滴含量。以上结果表明,10、20、40 nmo/L孕酮和地塞米松能够促进脂滴的形成,米非司酮抑制脂滴的形成,20、40、80 nmol/L雌二醇对脂滴的形成有促进作用,10 nmol/L雌二醇没有明显的差异性。
[Abstract]:Cervix is an important organ for female animals to maintain pregnancy and labor. The maturity of cervix is very important to the success of induced labor, the smooth delivery and the length of labor. Cervix maturation is regulated by many cytokines and hormones. It is a biochemical and inflammatory process. The uterine body is also an important reproductive organ of female animals. It is the place where the fetus is conceived. It plays an irreplaceable role in sperm migration, fertilized egg implantation and embryo, the growth, development and delivery of the fetus. The cervix contains fibroblasts, epithelial cells, endothelial cells and preadipocytes and adipocytes. Fibroblasts, derived from the mesoderm of embryonic stage, are one of the main cells in connective tissue. It has the ability of differentiation, participates in wound repair, induces osteogenesis and adipogenesis, and intertransforms with fibroblasts and mesenchymal stem cells under certain conditions. Adipose tissue can also be used as an endocrine organ to participate in collective physiological and pathological processes. Preadipocytes or adipocytes can secrete cytokines and hormones, and fat cells with large fat droplets have been found in the cervix of parturient goats. The presence of fat cells containing large lipid droplets was also found in the cervix of mice, and the number, distribution and changes of lipid droplets in the cervix of mice were studied accordingly, and some achievements were obtained. Lipid droplets were originally thought to be just particles for storing energy, similar to glycogen, an "inert" intracellular substance, but as the proteomics of lipid droplets progressed, researchers found, Lipid droplets are also a complex, dynamic, and active multifunctional organelle. It can move along the cytoskeleton and interact with other organelles, which may play a very important role in signal transduction, membrane transport, lipid storage and metabolism. Lipid droplets are widely found in adipocytes, hepatocytes, muscle cells and adrenal cortical cells. In this study, Kunming mice were used to study whether lipid droplets were affected by hormones in mouse cervix. The cervix of adult mice and mice treated with ovariectomized injection hormones (estradiol, progesterone, dexamethasone and mifepristone) were collected. Cultured in DMEM medium containing fetal bovine serum, and adding certain concentration of hormone to the medium, respectively, no hormone was found in serum. The concentrations of serum hormone free and serum excitins were 10 nmol / L ~ (20) nmol / L ~ (40) nmol / L ~ (80) nmol / L respectively, and oil red O staining was observed. The results showed that the positive rate in cervix of mice injected with progesterone and dexamethasone was higher than that of mice injected with mifepristone. The results of the other groups were consistent with those of the cervix. The lipid droplets in the cells cultured in the medium supplemented with progesterone were higher than those in the cells treated with mifepristone. The content of lipid droplets in the culture medium containing dexamethasone was also higher than that in the medium containing mifepristone. The results showed that progesterone and dexamethasone could promote the formation of lipid droplets. Mifepristone inhibited lipid droplet formation. Estradiol (estradiol) could promote the formation of lipid droplets. There was no significant difference between estradiol (estradiol) and estradiol (10nmol / L).
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S857.2

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