大肠杆菌O157∶H7 Ivy蛋白表位多肽及免疫原性分析

发布时间：2018-06-19 07:50

本文选题：肠出血性大肠杆菌O∶H + 脊椎动物溶菌酶抑制剂　；参考：《中国畜牧兽医》2017年01期

【摘要】：为评价合成的O157∶H7Ivy146—157多肽作为疫苗抗原的可行性,本试验利用生物信息学方法分析Ivy的二级结构、氨基酸亲水性、蛋白柔性区域、表面可及性和抗原指数,筛选获得1条含有B细胞抗原表位的多肽序列Ivy146—157,进行化学合成。多肽免疫BALB/c小鼠,测定免疫后小鼠的抗体水平及Ivy146—157多肽对小鼠脾淋巴细胞的刺激增殖情况。结果显示,合成的Ivy146—157多肽第3次免疫小鼠后抗体水平达到1∶6 400,制备的Ivy146—157多克隆抗体能够识别天然菌株中的目的蛋白;MTT试验结果显示,Ivy146—157免疫后小鼠脾淋巴细胞增殖明显,与对照组差异显著(P0.05)。结果表明,Ivy146—157多肽能够诱导机体产生体液免疫和细胞免疫反应,为其作为疫苗用抗原的进一步研究奠定了理论基础。
[Abstract]:In order to evaluate the feasibility of the synthesized O157: H7Ivy146-157 peptide as vaccine antigen, the secondary structure, amino acid hydrophilicity, protein flexibility, surface accessibility and antigen index of Ivy were analyzed by bioinformatics. A polypeptide sequence Ivy146-157 containing B cell epitope was selected and synthesized chemically. BALB / c mice were immunized with polypeptide. The antibody level and the proliferation of spleen lymphocytes stimulated by Ivy146-157 polypeptide were measured. The results show that The antibody level of the synthesized Ivy146-157 polypeptide reached 1:6 400 after the third immunization. The synthesized polyclonal antibody of Ivy146-157 could recognize the target protein in the natural strain. MTT test showed that the spleen lymphocyte proliferation of mice was obvious after immunization with Ivy 146-157. There was a significant difference between the control group and the control group (P 0.05). The results showed that the polypeptide of ivy 146-157 could induce humoral and cellular immune responses, which laid a theoretical foundation for the further study of Ivy 146-157 as a vaccine antigen.
【作者单位】：广西兽医研究所;广西兽医生物技术重点实验室;广西大学动物科学技术学院;
【基金】：广西自然科学基金(桂科自2014GXNSFAA118119) 广西科研基本业务费专项(桂科专项14-3)
【分类号】：S852.4

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