饲粮铜含量对牦牛体外瘤胃发酵的影响

发布时间：2018-06-19 09:20

本文选题：牦牛 + 甘氨酸铜　；参考：《动物营养学报》2016年08期

【摘要】：为了探究牦牛饲粮中微量元素铜的适宜含量,本试验以甘氨酸铜作为添加形式,以牦牛饲粮为底物进行体外瘤胃发酵,底物铜含量分别为5.0、10.0、15.0、20.0、25.0 mg/kg,共发酵48 h。测定发酵后的产气量、瘤胃发酵指标及消化酶活力。结果表明:当底物铜含量为15.0 mg/kg时,干物质消化率(DM D),微生物蛋白质(M CP)、丙酸、异丁酸、丁酸、异戊酸、戊酸、总挥发性脂肪酸浓度,脂肪酶(LPS)、胰蛋白酶(TYS)、纤维素酶(CLS)活力均达到最大值,分别为63.858%、4.289 g/L、24.475 mmol/L、0.470 mmol/L、8.977 mmol/L、1.159 mmol/L、1.607 mmol/L、81.583 mmol/L、0.504 U/m L、84.167 U/m L、79.956 U/m L,乙酸/丙酸最低为2.045;当铜含量为10.0 mg/kg时,M CP、乙酸浓度达到最大值,分别为4.289 g/L、51.075 mmol/L,且其他指标也都处于较高水平。综合得出,在体外条件下,牦牛饲粮推荐铜含量为10.0~15.0 mg/kg。
[Abstract]:In order to investigate the suitable content of trace element copper in yak diet, the rumen fermentation in vitro was carried out with copper glycine as a supplement and yak diet as substrate. The copper content of substrate was 5.010.0 (15.0 ~ 20.0) ~ 25.0 mg 路kg ~ (-1) 路kg ~ (-1), and the fermentation time was 48 h. After fermentation, gas production, rumen fermentation index and digestive enzyme activity were measured. The results showed that when the substrate copper content was 15.0 mg/kg, the dry matter digestibility was higher than DM DX, the microbial protein MCPN, propionic acid, isobutyric acid, isovaleric acid, total volatile fatty acid concentration. The activity of lipase, trypsin, cellulase (CLS) reached its maximum value, 63.858% 4.289 g / L 24.475 mmol / L 0.470 mmol / L / L 8.977 mmol / L 1.159 mmol / L 1.607 mmol / L 81.583 mmol / L = 84.504 U / L = 84.956 U / L = 2.045; when the copper content was 10.0 mg/kg, the concentration of acetic acid reached a maximum, and the concentration of acetic acid / propionic acid was as low as 2.045 when the copper content was 10.0 mmol / L = 1.159 mmol / L = 1.607 mmol / L = 81.583 mmol / L = 84.504 U / L = 84.956 U / L = 79.956 U / L = 2.045 mmol / L, respectively. It was 4.289 g / L, 51.075 mmol / L, and other indicators were at a high level. It was concluded that the recommended copper content in yak diet was 10.0 ~ 15.0 mg 路kg ~ (-1) 路kg ~ (-1).
【作者单位】：省部共建三江源生态与高原农牧业国家重点实验室青海省高原放牧家畜动物营养与饲料科学重点实验室青海高原牦牛研究中心青海大学畜牧兽医科学院;海北州畜牧兽医科学研究所;
【基金】：“973”国家重点基础研究发展计划项目(2012CB722906) “十二五”国家科技支撑计划项目(2012BAD13B01) 国家国际科技合作专项项目(2015DFG31870) 公益性行业(农业)科研专项(201203008) 教育部长江学者和创新团队发展计划项目(IRT13074) 青海省重大科技平台建设项目(2013-Z-Y03)
【分类号】：S823.5

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