禽白血病重组毒FJ15TH0株不同感染方式对SPF鸡感染状态及组织病毒载量的影响

发布时间：2018-06-19 09:56

  本文选题：重组ALV + 排毒　； 参考：《病毒学报》2017年05期

【摘要】：为揭示禽白血病病毒(ALV)自然重组毒FJ15TH0感染SPF鸡群的不同方式对其感染状态和病毒组织分布的影响,本研究设计动物试验分别模拟垂直传播和生长早中期水平传播,动态检测各组病毒血症、泄殖腔排毒、抗体反应及不同内脏组织病毒载量,为制定地方鸡群中ALV感染的防控和净化措施提供可靠的流行病学依据。结果表明胚内接种模拟垂直传播组除2只实验鸡外均产生免疫耐受,持续带毒排毒而无抗体产生;1日龄和D组均未表现泄殖腔排毒,在接触病毒后2~3w后C组63.6%实验动物表现一过性病毒血症,D组未表现病毒血症,暴露后5~11w期间C组9.1%~25%鸡出现一过性血清抗体,D组在暴露后3~5w期间14.3%~42.8%实验鸡产生一过性血清抗体。11w时剖杀实验鸡,荧光定量RT-PCR检测各组各实验鸡不同组织的gp85mRNA及病毒粒子的基因组RNA含量,结果显示垂直感染组表现泄殖腔排毒者gp85检测均为阳性,该分离株对肾脏和法氏囊的组织亲嗜性最强,而对肝脏和脾脏的亲嗜性较低;而同居感染组实验鸡均表现为gp85mRNA检测阴性,表明该重组毒水平感染能力较弱。
[Abstract]:In order to reveal the effects of different ways of FJ15TH0 infection on the infection status and tissue distribution of avian leukemia virus (ALV) FJ15TH0, this study designed animal experiments to simulate vertical transmission and horizontal transmission in early and middle stage of growth, respectively. Dynamic detection of viremia, cloaca detoxification, antibody response and viral load in different visceral tissues provided a reliable epidemiological basis for the prevention, control and purification of ALV infection in local chickens. The results showed that immune tolerance was produced in the simulated vertical transmission group except for two experimental chickens, and no cloacal detoxification was observed in group D and no antibody was produced at 1 day after inoculation. 63.6% of the animals in group C showed transient viremia and group D did not show viremia at 2 weeks after exposure to the virus, and 63.6% of the animals in group C showed transient viremia after exposure to the virus. Transient serum antibody was found in group C (9.1g / 25%) during the period of 5w / 11w after exposure. In group D, 14.32.8% chickens produced transient serum antibodies (TSA) during 5 weeks after exposure. Fluorescence quantitative RT-PCR was used to detect the content of gp85 mRNA and genomic RNA of virus particles in different tissues of different chickens in each group. The results showed that the gp85 detection was positive in the patients with vertical infection, and the isolate had the strongest affinity to the kidney and bursa of Fabricius. In the cohabitation group, the gp85 mRNA was negative, which indicated that the recombinant virus level was weak.
【作者单位】： 福建农林大学动物科学学院中西兽医结合与动物保健福建省高校重点实验室;
【基金】：福建省自然科学基金面上项目(项目号:2014J01072),题目:env基因变异对血管瘤型J亚群禽白血病病毒致瘤特性的影响~~
【分类号】：S858.31
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本文编号：2039488