禽腺病毒4型感染病理组织学观察

发布时间：2018-06-20 06:18

本文选题：禽腺病毒型 + 鸡　；参考：《中国兽医学报》2017年09期

【摘要】：将30只1日龄SPF鸡随机分为2组,其中攻毒组20只,对照组10只。攻毒组按每只0.2mL腹腔注射禽腺病毒4型(FAV-4)病毒液,对照组不做处理,隔离饲养,临床观察2周。攻毒后第3天攻毒组开始出现死亡,将死亡鸡解剖观察各器官大体病变,经聚合酶链式反应(PCR)检测为FAV-4后,采集心脏、肝脏、脾脏、肾脏、肺脏制作石蜡切片,观察病理组织学变化。结果显示,攻毒组死亡鸡只无明显临床症状而突然倒地死亡;主要剖检病变为心包积液、肝脏肿大,个别可出现脾脏肿大、肺脏淤血、肾脏肿大;病理组织学变化为肝脏、肺脏、肾脏淤血,细胞变性坏死;而对照组无任何异常症状和病理组织学变化。
[Abstract]:Thirty 1-day SPF chickens were randomly divided into 2 groups, 20 in the drug attack group and 10 in the control group. Avian adenovirus type 4 (FAV-4) was injected intraperitoneally in the control group (0.2 mL), while the control group was kept in isolation and kept in isolation for 2 weeks. On the 3rd day after poisoning, the dead chickens were dissected to observe the gross changes of the organs. After the detection of FAV-4 by polymerase chain reaction (PCR), the hearts, liver, spleen, kidneys and lungs were collected to make paraffin sections. Histopathological changes were observed. The results showed that the dead chickens in the drug attack group fell to the ground suddenly without obvious clinical symptoms. The main pathological changes were pericardial effusion, hepatomegaly, splenomegaly, pulmonary congestion and kidney enlargement. The pathological changes were liver. There were no abnormal symptoms and histopathological changes in the control group.
【作者单位】：河北农业大学动物医学院;河北省兽医生物技术工程技术研究中心;
【基金】：河北省现代农业产业技术体系蛋鸡产业创新团队专项资金资助项目(HBCT2013090202) 河北省高校百名优秀创新人才支持计划资助项目(Ⅲ)(SLRC2017039) 2016年河北农业大学大学生创新创业训练计划项目河北省大学生创新创业训练计划资助项目(201610086055)
【分类号】：S858.31

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