三个绵羊品种BMPR-IB基因CDS区1113位点的多态性与产羔数的相关性分析

发布时间：2018-06-20 17:16

本文选题：骨形态发生蛋白受体IB基因(BMPR-IB) + 小尾寒羊　；参考：《农业生物技术学报》2017年12期

【摘要】：绵羊(Ovis aries)的多胎性状由主效基因控制,骨形态发生蛋白受体IB基因(bonemorphogenetic protein type IB,BMPR-IB)已被证实为多产母羊的主效基因之一。为寻找绵羊繁殖力的标记和育种提供理论依据,本研究采用聚合酶链反应-单链构象多态性分析法(single strand conformation polymorphism analysis of polymerase chain reaction products,PCR-SSCP)对3种绵羊(小尾寒羊48只;蒙古羊188只,其中包括100只单胎母羊,88只双胎母羊;甘肃高山细毛羊112只,其中包括70只单胎母羊,42只双胎母羊)BMPR-IB基因编码区1113位点进行单核苷酸多态性(single nucleotide polymorphism,SNP)检测,并分析3种绵羊BMPR-IB基因的多态性及其与产羔数之间的关联性,以期为研究绵羊高繁殖力的机理提供资料。结果表明,在蒙古羊单、双胎母羊和甘肃高山细毛羊单、双胎母羊中均发现3种不同基因型,分别为野生型AA、纯合突变型BB和杂合突变型AB,优势基因型为AB;小尾寒羊出现两种基因型,分别为纯合突变型BB和杂合突变型AB,优势基因型为BB;经多态信息含量(polymorphism information content,PIC)分析可知,小尾寒羊处于低度多态(PIC0.25),甘肃高山细毛羊和蒙古羊均处于中度多态(0.25PIC0.5);BMPR-IB基因编码区1113位点突变在不同繁殖力绵羊品种间存在显著差异(P0.05),该位点可能与绵羊产羔数有关,可作为繁殖性状的标记位点,本研究结果可进一步为生产实践提供理论依据。
[Abstract]:The reproductive traits of Ovis aries are controlled by major genes. Bone morphogenetic protein type IBB (BMPR-IBR) has been proved to be one of the major genes in the fecundity of ewes. In order to provide theoretical basis for identification and breeding of sheep fecundity, polymerase chain reaction-single-strand conformation polymorphism analysis (PCR-SSCP) was used to study three kinds of sheep (48 small tail Han sheep and 188 Mongolian sheep). Among them, 88 single ewe and 112 Gansu Alpine fine wool sheep, including 70 single ewe, 42 twin ewe, 1113 site of BMPR-IB gene coding region, were detected by single nucleotide polymorphism single nucleotide polymorphismSNPs (SNPs). The polymorphism of BMPR-IB gene and the relationship between BMPR-IB gene polymorphism and litter size in three sheep were analyzed in order to provide data for the study of the mechanism of high fecundity of sheep. The results showed that three different genotypes were found in Mongolian single, twin ewe, Gansu alpine fine wool sheep and twin ewe. They were wild type AAA, homozygous mutant BB and heterozygous mutant ABB, dominant genotype ABand small tail Han sheep with homozygous mutant BB and heterozygous mutation, respectively. The dominant genotype was BBB.The polymorphism information polymorphism (Pi) analysis showed that there were two genotypes in small tail Han sheep, and the results showed that there were two genotypes in small tail Han sheep, which were homozygous BB and heterozygous mutation, respectively. Small tail Han sheep were in low polymorphic PIC0.25, Gansu alpine fine wool sheep and Mongolian sheep were both in moderate polymorphism 0.25PIC0.5BPR-IB gene coding region 1113 mutation in different fecundity sheep breeds, this position may be related to sheep litter size. The results can be used as marker loci for reproductive traits and provide theoretical basis for production practice.
【作者单位】：甘肃农业大学动物科学技术学院;
【基金】：甘肃农业大学科研项目(No.GSAU-ZL-2015-027) 国家自然科学基金(No.31260547)
【分类号】：S826

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