大肠杆菌O抗原定型血清标定用抗原的制备

发布时间：2018-06-21 01:56

本文选题：大肠杆菌 + 菌体抗原　；参考：《中国兽药杂志》2016年12期

【摘要】：为研制用于标定大肠杆菌菌体抗原(O抗原)单因子定型血清效价的抗原,以大肠杆菌参考菌株CVCC1345(O2)、CVCC1350(O7)、CVCC1414(O74)为菌种,经条件优化确定了抗原制备的工艺。检测结果表明,当菌液调整至OD_(600 nm)值为0.147±0.026时,各型抗原的浓度为1×10~9CFU/m L,将其热处理后制备成反应抗原。按此吸光值各自制备3批抗原,3批抗原与血清的凝集价均分别一致,分别达到2~(11)(O2)、2~(12)(O7)及2~9(O74)。用180种大肠杆菌O抗原单因子定型血清对制备的抗原进行检测,制备的抗原仅与其对应型的血清发生特异性反应,与其余179种血清均不发生反应。研究结果显示,制备的抗原具有较好的稳定性及特异性,可以用于大肠杆菌菌体单因子定型血清效价的标定。
[Abstract]:In order to prepare the antigen used to calibrate the single factor serotactic titer of Escherichia coli antigen O, a reference strain of E. coli CVCC1345O2, CVCC1350O7, CVCC1414O74, was used as the strain. The preparation process of the antigen was determined by optimizing the conditions. The results showed that the concentration of each antigen was 1 脳 10 ~ (9) CFU / m ~ (-1), and the reaction antigen was prepared after heat treatment when the bacterial liquid was adjusted to ODV (600nm) = 0.147 卤0.026, and the concentration of each antigen was 1 脳 10 ~ (9) CFU / m ~ (-1). According to the absorptivity values, the agglutination values of three batches of antigens and three batches of antigens were the same as those of the serum, which were up to 2C / 11C ~ (2 / O _ (2) O _ (2) O ~ (2 +) and 2 ~ (9) O _ (74) ~ (7) respectively. The antigens were detected with 180 kinds of single factor serums of Escherichia coli O antigen. The antigens were only specific to the corresponding sera, but not to the other 179 kinds of sera. The results showed that the prepared antigen had good stability and specificity, and could be used to calibrate the titer of single factor serotonin of Escherichia coli.
【作者单位】：中国兽医药品监察所;
【分类号】：S852.61

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