水貂阿留申病病毒NS1蛋白B细胞抗原表位的分析与鉴定

发布时间：2018-06-21 07:37

本文选题：水貂阿留申病病毒 + NS蛋白　；参考：《中国兽医科学》2016年02期

【摘要】：为了鉴定水貂阿留申病病毒(AMDV)NS1蛋白的抗原表位,利用生物信息学软件对NS1蛋白的理化性质、二级结构、三级结构及抗原性进行综合分析,预测结果显示,35~56aa、134~143aa、273~288aa、302~321aa、387~395aa、499~509aa存在抗原表位的可能性较大。采用固相合成法合成6条候选表位肽,将其作为包被抗原,利用水貂阿留申病病毒阴性血清及阳性血清,采用间接ELISA检测方法验证候选表位肽的反应原性,结果显示合成的6条候选表位肽的反应原性均较好,其中302~321aa对AMDV抗体识别的特异性较高,与对流免疫电泳技术(CIEP)检测方法的符合率可达86.7%,并能够检测出部分CIEP检测呈阴性而PCR检测呈阳性的感染早期血样。本研究为水貂阿留申病病毒多肽检测抗原开发及诊断方法的建立奠定了基础。
[Abstract]:In order to identify the antigenic epitopes of the NS1 protein of mink Aleutian disease virus, the physicochemical properties, secondary structure, tertiary structure and antigenicity of NS1 protein were comprehensively analyzed by bioinformatics software. The predicted results showed that the antigenic epitopes were more likely to be present in 35A5 ~ 56aAU ~ (134) ~ 143aa ~ (1) ~ (27) ~ (28) ~ (28) A ~ (a) ~ (3) ~ (3) ~ (21) ~ (3) ~ (21) A ~ (-1) ~ (3 821) A ~ (3 +) ~ ( Six candidate epitope peptides were synthesized by solid phase synthesis and used as coated antigens. The reactivity of candidate epitope peptides was verified by indirect Elisa with negative and positive sera of Aleutian disease virus of mink. The results showed that all of the 6 candidate epitope peptides synthesized were highly reactive, and the specificity of 302~321aa to the recognition of AMDV antibody was higher. The coincidence rate of CIEP with CIEP was 86.7, and some of the early blood samples with negative CIEP and positive PCR could be detected. This study laid a foundation for the development of antigen detection and the establishment of diagnostic method for Aleutian disease virus polypeptide in mink.
【作者单位】：中国农业科学院特产研究所特种经济动物分子生物学国家重点实验室;吉林农业大学动物科学技术学院;
【基金】：吉林省科技发展计划项目(201201001) 吉林省科技攻关计划项目(20140204074NY)
【分类号】：S852.65

【相似文献】