不同动物源肠球菌耐药性及毒力基因测定与分析

发布时间：2018-06-21 19:42

本文选题：动物 + 肠球菌　；参考：《中国人兽共患病学报》2017年11期

【摘要】：目的通过测定120株不同来源肠球菌耐药性分布情况,毒力基因cylA、efaA、gelE、esp、ace和AS的携带率,旨在了解肠球菌耐药现状和毒力基因分布情况,为选择临床治疗方案和探索肠球菌致病机制提供参考。方法设计6对特异性引物,应用PCR方法对分离到的120株肠球菌进行6种毒力基因检测,同时用纸片扩散法对11种常用抗生素进行耐药性试验。结果 120个肠球菌分离株对抗生素均具有3重以上的耐药性,且不同来源菌株的多重耐药性有明显差异,其中以病死猪内脏分离株多重耐药性最为严重;总体上,上述分离株对林可霉素耐药率最高,为达96.00%(115/120),对环丙沙星、卡那霉素、四环素和达福普汀的耐药率均达到50%以上,而对替考拉宁的耐药率最低,仅为0.83%(1/120)。6种毒力基因cylA、efaA、gelE、esp、ace和AS在受试菌株中的阳性检出率依次为39.17%、56.67%、68.33%、23.33%、26.67%和13.33%;不同来源的肠球菌毒力基因携带率不同,且携带毒力基因数量也有明显差异,其中,来自病死猪内脏的肠球菌分离株携带毒力基因的比例较高,且有超过50%的菌株同时携带4种毒力基因及以上。结论肠球菌的耐药率和多重耐药性与毒力基因携带阳性率有一致性,且与菌株来源关系密切,因此对这一传统意义上的机会性病原体值得深入研究其中的因果关系。
[Abstract]:Objective to determine the distribution of drug resistance of 120 strains of Enterococcus from different sources, and the carrying rate of virulence gene cylAfida Eel Eespneace and as in order to understand the present situation of drug resistance and the distribution of virulence genes of Enterococcus. To provide reference for selecting clinical treatment and exploring pathogenic mechanism of Enterococcus. Methods six pairs of specific primers were designed and 6 virulence genes of 120 strains of Enterococcus were detected by PCR method. Meanwhile, drug resistance of 11 common antibiotics was tested by disk diffusion method. Results all the 120 strains of Enterococcus had three or more antimicrobial resistance to antibiotics, and the multidrug resistance of the strains from different sources were significantly different, among which the most serious was the swine visceral isolates. The resistance rate of the above isolates to lincomycin was the highest, reaching 96.00 / 115 / 120. The resistance rates to ciprofloxacin, kanamycin, tetracycline and dafoputin were all over 50%, while those to teicoplanin were the lowest. The positive rates of cylAfen Egel Eespneace and as were 39.17% and 13.33%, respectively. The virulence rate of enterococcus virulence genes from different sources was different and the number of virulence genes was also different, among them, the number of virulence genes was also different from that of enterococcal enterococci, and the number of virulence genes was also significantly different from that of different sources of enterococci, and the positive rate was 39.17.1% and 13.33% respectively, and there were significant differences in the number of virulence genes carried by enterococcus virulence genes. The proportion of virulence genes of Enterococcus isolated from dead pig viscera was high, and more than 50% of the strains carried four virulence genes and more than 4 virulence genes simultaneously. Conclusion the drug resistance rate and multidrug resistance of Enterococcus are consistent with the positive rate of virulence gene carrying, and are closely related to the source of the strain. Therefore, the causality of the opportunistic pathogen in the traditional sense is worthy of further study.
【作者单位】：河南农业大学牧医工程学院;河南省动物性食品安全重点实验室;
【基金】：河南省重点科技攻关项目(No.152102310081)~~
【分类号】：S852.61

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