猴源宋内氏志贺菌的分离鉴定及生物学特性分析

发布时间：2018-06-22 22:24

本文选题：宋内氏志贺菌 + 猕猴　；参考：《中国兽医科学》2017年01期

【摘要】：从四川省某规模化猕猴养殖基地的病死猕猴的脏器及肠道内容物中分离到1株可疑菌,对其纯化培养、革兰染色和生化鉴定后进行了分子水平鉴定,包括ipa H基因和wzy基因测定、16S r RNA基因序列分析,并进行了致病性分析及药敏试验。结果显示,该分离菌符合宋内氏志贺菌生物学及分子学特性,对小鼠具有较强致病性。16S r RNA基因多序列比对,绘制遗传距离及遗传发育树,证明其与2株宋内氏志贺菌(CP010829.1和HE616528.1)遗传距离最小,均为0.001。药敏试验显示,该菌株对头孢类等4类药物敏感,对青霉素类等6类药物耐药。结果表明,该分离株为宋内氏志贺菌且具有较强的致病性和耐药性。
[Abstract]:A strain of suspicious bacteria was isolated from organs and intestinal contents of diseased and dead rhesus monkeys in a large-scale breeding base of rhesus mulatta in Sichuan Province. The bacteria were purified, cultured, and identified by Gram staining and biochemical identification at molecular level. The sequence analysis of 16s r RNA gene of ipa H gene and wzy gene was carried out, and the pathogenicity analysis and drug sensitivity test were carried out. The results showed that the isolated strain accorded with the biological and molecular characteristics of Shigella sonnei, and had strong pathogenicity. 16s r RNA gene multiple sequence alignment, genetic distance and genetic development tree were plotted. It was proved that the genetic distance between the two strains of Shigella sonnei (CP010829.1 and HE616528.1) was the smallest, which was 0.001. The drug sensitivity test showed that the strain was sensitive to four kinds of drugs such as cephalosporins and was resistant to 6 kinds of drugs such as penicillin. The results showed that the strain was Shigella sonnei and had strong pathogenicity and resistance.
【作者单位】：四川农业大学动物医学院实验动物疾病模型研究室;四川农业大学动物医学院动物疾病与人类健康中心四川省重点实验室;
【基金】：国家科技支撑计划资助项目(2014BAI03B01) 国家重大科学仪器设备开发专项资助项目(2013YQ49085906)
【分类号】：S852.61

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