复方芪术颗粒对小鼠脾淋巴细胞增殖及雏鸡疫苗免疫效果的影响

发布时间：2018-06-23 02:42

本文选题：复方芪术颗粒 + 淋巴细胞　；参考：《中国家禽》2017年08期

【摘要】：为探讨复方芪术颗粒的免疫调节作用,分别进行复方芪术颗粒对小鼠脾淋巴细胞增殖及鸡新城疫(ND)、传染性法氏囊病(IBD)疫苗血清抗体产生水平影响的研究。在小鼠脾淋巴细胞增殖试验中,复方芪术颗粒低、中、高剂量组给药剂量分别为3.75、7.5、15 g/kg;在对ND、IBD疫苗血清抗体产生水平影响的研究中,黄芪多糖阳性药物组(7.5 g/kg)以及复方芪术颗粒低、中、高剂量组给药剂量分别为5、7.5、10 g/kg。结果表明:复方芪术颗粒能在以下三方面发挥调节作用:(1)提高免疫低下小鼠脾淋巴细胞的增殖活性,其中低剂量组OD值显著高于环磷酰胺组(P0.05);(2)提高ND疫苗血清抗体产生水平,在首免后7 d低剂量组鸡新城疫病毒抗体水平显著高于空白组(P0.05),二免后7 d低剂量组的抗体水平极显著高于空白组(P0.01),中剂量组显著高于空白组(P0.05);(3)提高IBD疫苗血清抗体产生水平,二免后7 d低剂量、中剂量组的传染性法氏囊病病毒抗体水平显著高于空白组(P0.05)。综上所述,复方芪术颗粒能调节机体的细胞免疫和体液免疫,增强特异性免疫功能。
[Abstract]:In order to study the immunomodulatory effect of compound Qizu granule on spleen lymphocyte proliferation and serum antibody production level of Newcastle disease (ND) and infectious bursal disease (IBD) vaccine in mice. In the spleen lymphocyte proliferation test of mice, the dosage of compound Qizhi granules was 3.75 ~ 7.5g / kg in low, medium and high dose groups, respectively. The dosage of Astragalus polysaccharide positive drug group (7.5 g/kg) and compound astragalus granule was lower than that of high dose group, and the dosage of Astragalus polysaccharide positive drug group was 5 ~ 7.5g / kg. The results showed that compound Qizu granule could play a regulatory role in the following three aspects: (1) the proliferative activity of spleen lymphocytes in immunocompromised mice was increased, and the OD value of low dose group was significantly higher than that of cyclophosphamide group (P0.05); (2), and the level of serum antibody production of ND vaccine was significantly higher in low dose group than in cyclophosphamide group. The antibody level of Newcastle disease virus in the low dose group was significantly higher than that in the blank group 7 days after the first immunization (P0.05), the antibody level in the low dose group was significantly higher than that in the blank group on the 7th day after the second immunization (P0.01), and the level of serum antibody in the middle dose group was significantly higher than that in the blank group (P0.05); (3). The antibody level of infectious bursal disease virus in the middle dose group was significantly higher than that in the blank group at 7 d after the second immunization (P0.05). To sum up, compound Qizu granule can regulate the cellular and humoral immunity and enhance the specific immune function.
【作者单位】：扬州大学兽医学院;四川维尔康动物药业有限公司;西南大学荣昌校区;
【基金】：隆昌县科技支撑计划专项资金江苏省高校优势学科建设工程资助项目
【分类号】：S853.7

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