脱氧雪腐镰刀菌烯醇对仔猪海马神经细胞的毒性作用

发布时间：2018-06-23 11:48

本文选题：脱氧雪腐镰刀菌烯醇 + 海马神经细胞　；参考：《中国兽医科学》2017年01期

【摘要】：为了研究脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)对仔猪海马神经细胞的毒性作用,于体外培养仔猪海马神经细胞至对数生长期,以0、125、250、500、1 000和2 000 ng/m L不同质量浓度的DON对细胞进行染毒处理,应用倒置显微镜和透射电镜观察细胞的形态学变化;Hoechest 33258染色液对细胞核进行标记,观察细胞核的形态学变化;CCK-8试剂盒检测细胞的存活率、乳酸脱氢酶(LDH)试剂盒检测LDH漏出率。结果显示,与对照组相比,处理组细胞发生了明显的形态学变化,随着DON质量浓度增加,细胞密度降低、空泡化、染色质浓缩聚集、产生凋亡小体;处理组细胞的存活率明显降低,具有剂量和时间依赖性。LDH漏出率随着DON质量浓度的增加而增加,与对照组相比,当质量浓度达到500 ng/m L以上时,差异极显著(P0.01)。试验表明,DON对仔猪海马神经细胞具有明显的毒性作用,能够诱导细胞损伤,发生凋亡。
[Abstract]:In order to study the toxicity of deoxynivalenoldol (deoxynivalenoldon) on hippocampal nerve cells of piglets, cultured hippocampal neurons in vitro to logarithmic growth stage, the cells were treated with don of different concentrations of 0 125 ng/m / L and 2 000 ng/m / L, respectively. The morphologic changes of cells were observed by inverted microscope and transmission electron microscope. The cell nucleus was labeled with Hoechest 33258 staining solution. The cell survival rate was detected by CCK-8 kit, and the leakage rate of LDH was detected by lactate dehydrogenase (LDH) kit. The results showed that compared with the control group, the cells in the treatment group had obvious morphological changes. With the increase of don concentration, cell density decreased, vacuolation, chromatin concentration and aggregation, and apoptotic bodies were produced. The survival rate of the cells in the treatment group was significantly decreased, and the leakage rate of LDH was increased with the increase of don concentration in a dose-and time-dependent manner. The difference was very significant when the concentration was above 500 ng/m / L compared with the control group (P0.01). The results showed that don had obvious toxic effect on hippocampal nerve cells of piglets and could induce cell damage and apoptosis.
【作者单位】：安徽农业大学动物科技学院;
【基金】：国家自然科学基金项目(31472250) 安徽省生猪产业技术体系资助项目(2016-2020)
【分类号】：S859.8

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