2016年～2017年我国华北地区某规模化猪场经典美洲型猪繁殖与呼吸综合征病毒的分离鉴定及全基因组序列分析

发布时间：2018-06-23 13:33

本文选题：经典猪繁殖与呼吸综合征病毒 + 病毒分离鉴定　；参考：《中国预防兽医学报》2017年09期

【摘要】：为了解近期我国华北地区猪繁殖与呼吸综合征病毒(PRRSV)的流行及变异情况,本研究对该地区某猪场采集33份2016年~2017年疑似患PRRS猪病料样品进行PCR检测,对阳性样品进行病毒分离,并从分离株中选择两株病毒(SD158和SD192)进行全基因的序列扩增及分析。结果显示,检测到13份PRRSV阳性病料样品,病原为经典美洲型PRRSV,但仅分离到11株病毒(4株感染MARC-145细胞,另外7株感染PAM细胞)。SD158和SD192株与Resp PRRS MLV株(疫苗株)的核苷酸同源性最高,分别为99.7%和99.4%。对这两株PRRSV与VR-2332、Resp PRRS MLV的各基因的推导氨基酸序列进行分析,结果显示在VR-2332与Resp PRRS MLV株22处差异氨基酸中,SD158株有15处、SD192株有14处氨基酸和Resp PRRS MLV株相同,但二者仅各有6处及7处氨基酸与VR-2332株一致,在鉴别PRRSV VR-2332株与Resp PRRS MLV株的7处可能与毒力相关的氨基酸位点时发现,SD158与SD192株各有5处基酸与VR-2332株相同。根据以上结果推测SD158与SD192株可能来源于PRRSV Resp PRRS MLV株,具有上述分子变化特征的PRRSV在我国的流行状况值得关注。
[Abstract]:In order to understand the recent prevalence and variation of porcine reproductive and respiratory syndrome virus (PRRSv) in North China, 33 samples of suspected PRRS from 2016 to 2017 were collected from a pig farm in this area, and the positive samples were isolated by PCR. Two viruses (SD158 and SD192) were selected from the isolates to amplify and analyze the whole gene. The results showed that 13 samples of PRRSv positive venereal diseases were detected, the pathogen was classic American PRRSV.However, only 11 strains of virus (4 strains infected with MARC-145 cells and 7 strains infected with PAM cells), SD158 and SD192 had the highest nucleotide homology with Resp PRRS MLV strain (vaccine strain), but only 11 strains (4 strains infected with MARC-145 cells and 7 strains infected with PAM cells) had the highest nucleotide homology with Resp PRRS MLV strain (vaccine strain). 99.7% and 99.4%, respectively. The deduced amino acid sequences of the two strains of PRRSV and VR-2332Resp PRRS MLV were analyzed. The results showed that 15 of the 22 different amino acids in the VR-2332 and Resp PRRS MLV strains were found to have the same amino acids in 15 of the SD192 strains and 14 of the Resp PRRS MLV strains. However, there were only 6 amino acids and 7 amino acids in each of them, which were identical with VR-2332 strains. In identifying 7 amino acid sites of PRRSV VR-2332 strain and Resp PRRS MLV strain, it was found that there were 5 amino acid sites in SD158 and SD192 strains, respectively, which were the same as VR-2332 strains. According to the above results, it is inferred that SD158 and SD192 strains may originate from PRRSv Resp PRRS MLV strain. The epidemic situation of PRRSv with the above molecular variation characteristics in China should be paid attention to.
【作者单位】：中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室;南阳师范学院;山东信得生物科技有限公司;中国农业科学院上海兽医研究所;
【基金】：国家自然科学基金(31270045、31502097)
【分类号】：S852.651

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