两种猪肌内脂肪含量测定方法的比较及其与胴体和肉质性状的相关性分析

发布时间：2018-06-24 12:19

本文选题：肌内脂肪 + 甲醇-氯仿法　；参考：《南京农业大学学报》2017年01期

【摘要】：[目的]比较分析甲醇-氯仿法(JL法)和索氏抽提法(SS法)提取肌内脂肪的效果,研究猪肌内脂肪含量与胴体质量、背膘厚、嫩度的相关与回归关系。[方法]屠宰测定了279头四元(皮杜长大)商品猪的胴体质量、背膘厚和肉嫩度,并利用索氏抽提法测定肌内脂肪含量,对肌内脂肪含量与其他性状指标进行相关性、回归及通径分析。随机挑选92份肉样,采用甲醇-氯仿法重新测定肌内脂肪含量,并与相应样本的索氏抽提法所得数据进行相关性与差异显著性分析。[结果]索氏抽提法测得的肌内脂肪湿含量和干含量之间极显著相关(r=0.996,P0.01);甲醇-氯仿法和索氏抽提法测得的肌内脂肪湿含量之间也极显著相关(r=0.909,P0.01),但索氏抽提法测得的肌内脂肪含量极显著高于甲醇-氯仿法(P0.01)。相关性分析结果显示,肌内脂肪含量与胴体质量、背膘厚及嫩度均存在极显著相关性(P0.01),背膘厚与胴体质量也存在极显著相关性(r=0.629,P0.01),但肌肉嫩度与胴体质量、背膘厚无显著相关性(P0.05)。回归分析结果表明:背膘厚和肉嫩度与肌内脂肪含量存在极显著的回归关系(P0.01),而胴体质量与肌内脂肪含量不存在显著的回归关系(P0.05)。[结论]索氏抽提法和甲醇-氯仿法均可作为有效测定肌内脂肪含量的方法,但在比较品种或个体间肌内脂肪含量差异时,需要使用同一种测定方法获得的数据;肌内脂肪含量与背膘厚、嫩度均存在显著相关和回归关系。
[Abstract]:[objective] to compare and analyze the effects of methanol-chloroform (JL) and Soxhlet extraction (SS) on the extraction of intramuscular fat, and to study the correlation and regression relationship between the content of intramuscular fat and carcass weight, backfat thickness, tenderness. [methods] carcass weight, backfat thickness and meat tenderness of 279 quaternary (Piedu) commercial pigs were measured by slaughter, and intramuscular fat content was measured by Soxhlet extraction method. Regression and path analysis. Ninety-two meat samples were randomly selected and the content of intramuscular fat was redetermined by methanol-chloroform method, and the correlation and difference were analyzed with the data obtained by Soxhlet extraction method. [results] there was a significant correlation between the wet and dry contents of intramuscular fat measured by Soxhlet extraction method (r = 0.996g, P 0.01), and a significant correlation between the wet content of intramuscular fat measured by methanol-chloroform method and Soxhlet extraction method (r = 0.909, P 0.01), but by Soxhlet extraction method, it was found that there was a significant correlation between the wet content of intramuscular fat and the content of dry fat by Soxhlet extraction method. The content of intramuscular fat was significantly higher than that of methanol-chloroform method (P0.01). The results of correlation analysis showed that there was a significant correlation between intramuscular fat content and carcass weight, backfat thickness and tenderness (P0.01), and between backfat thickness and carcass quality (r = 0.629, P0.01), but there was no significant correlation between muscle tenderness and carcass weight and backfat thickness (P0.05). Regression analysis showed that there was a significant regression relationship between back fat thickness and meat tenderness and intramuscular fat content (P0.01), but there was no significant regression relationship between carcass weight and intramuscular fat content (P0.05). [conclusion] Soxhlet extraction method and methanol-chloroform method can be used as effective methods for the determination of intramuscular fat content. There was a significant correlation and regression relationship between intramuscular fat content and backfat thickness and tenderness.
【作者单位】：南京农业大学动物科技学院;海南省农业科学院畜牧兽医研究所;
【基金】：中央高校基本科研业务费专项资金项目(KYCYL201502-1,KJQN201605) 海南省家畜家禽工程技术研究中心项目(XQ2016JSZX001) 国家自然科学青年基金项目(31501920)
【分类号】：S828

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