产后奶牛子宫内致病菌的分离、毒力基因检测及耐药性分析

发布时间：2018-06-25 04:25

本文选题：奶牛 + 子宫内膜炎　；参考：《中国农业大学学报》2017年02期

【摘要】：为检测产后奶牛子宫内膜炎致病菌的毒力基因和耐药性,在生产3周后采用棉拭子法对65头确诊的临床型子宫内膜炎荷斯坦奶牛进行子宫黏液的采集;分别用阴门灌注大鼠子宫试验、PCR方法以及药敏纸片法来检验分离株的致病性。结果表明,灌注混合菌(1∶1,1.0×10~9 cfu/mL)72h后剖解大鼠子宫发现子宫充血,肿大,增厚,子宫内部有蓄脓现象。然而,Escherichia coli和Staphylococcus aureus从患病牛中最终检出率分别为58.69%、62.75%;并对27株致病性E.coli系统发育组ChuA、yjaA基因检出率达到(13/27,48.15%),且E.coli和S.aureus分离株中均含有毒力基因KpSMII和TSST-1;随机抽取10头患病牛且含毒力基因最多的分离株进行药敏试验且均耐受于青霉素、多西环素、多粘菌素B、阿莫西林和四环素等抗生素。由此可见,以上抗生素再继续应用将会导致E.coli和S.aureus耐药程度持续增加。
[Abstract]:In order to detect the virulence genes and drug resistance of endometritis pathogens in postpartum cows, 65 clinical endometritis Holstein cows were collected by cotton swab method after 3 weeks of production. The pathogenicity of the isolated strain was tested by PCR method and drug sensitive disk method. The results showed that hyperemia, swelling and thickening of uterus were found in the uterus of rats after 72 hours of dissection of mixed bacteria (1: 1 0 脳 10 9 cfur / mL), and there was a phenomenon of accumulation of pus in the uterus. However, the final detection rates of Escherichia coli and Staphylococcus aureus from infected cattle were 58.69 and 62.75, respectively, and the detection rate of ChuAYjaA gene in 27 strains of pathogenic E. coli phylogenetic group reached (13 / 2748.15%), and the virulence genes KpSMII and TSST-1 were found in E. coli and S. aureus isolates, and 10 infected cattle were randomly selected. The isolates with the most virulence genes were tested for drug sensitivity and were tolerant to penicillin. Antibiotics such as doxycycline, polymyxin B, amoxicillin and tetracycline. Therefore, the continued use of the above antibiotics will result in a continuous increase in resistance to E. coli and S. aureus.
【作者单位】：内蒙古农业大学兽医学院;内蒙古农业大学动物科技学院;
【基金】：国家自然科学基金项目(31260590) 国家科技支撑项目(2015BAD29B06)
【分类号】：S858.23

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