谷胱甘肽过氧化物酶-5在绵羊附睾的表达

发布时间：2018-06-27 22:19

本文选题：附睾 + 精子　；参考：《内蒙古农业大学》2015年硕士论文

【摘要】：谷胱甘肽过氧化物酶-5 (Glutathione peroxidase type-5)在动物附睾中特异性表达,并对精子的成熟起着至关重要的作用。本文研究了GPX5在不同年龄的绵羊附睾中的表达情况。研究结果如下：1.通过实时荧光定量PCR,检测GPX5基因在不同年龄绵羊附睾,睾丸和输精管的相对表达量。结果表明GPX5基因在幼龄(2-3月龄)羔羊附睾、睾丸和输精管中几乎不表达；而在青年(6-12月龄)绵羊附睾头,GPX5基因的表达量极显著高于附睾体和附睾尾(p0.01),在输精管和睾丸几乎不表达；在成年(2-3岁)绵羊附睾头显著高于附睾体和附睾尾(p0.05),但显著低于青年绵羊附睾头的表达量。GPX5在不同年龄绵羊的睾丸和输精管的表达量没有显著差异。2.通过western blot的方法检测不同年龄绵羊的附睾和睾丸中GPX5蛋白的表达。结果显示在羔羊附睾和睾丸未检测到GPX5蛋白；在青年绵羊附睾头高表达,附睾体和附睾尾中低表达,睾丸中不表达；在成年绵羊附睾头少量表达,附睾体,附睾尾和睾丸几乎都不表达。3.利用免疫组化的方法,对GPX5蛋白在附睾上进行定位。经过免疫组化分析,GPX5蛋白主要在附睾头上皮细胞质以及静纤毛表达,附睾体和附睾尾中的GPX5蛋白主要集中在附睾管腔中,上皮少量表达。并且附睾尾的GPX5蛋白主要在静纤毛表达。GPX5蛋白从附睾上皮分泌到附睾腔中,随着精子在附睾中的运输与精子结合。总之,GPX5在不同年龄的绵羊附睾中的表达存在差异。GPX5主要在青年绵羊和,成年绵羊的附睾头上皮表达,GPX5蛋白分泌到附睾管腔中与精子结合,保护精子在附睾运输过程中不受活性氧(ROS)的攻击,保证精子功能的正常发挥。
[Abstract]:Glutathione peroxidase -5 (Glutathione peroxidase type-5) is specifically expressed in animal epididymis and plays a vital role in the maturation of sperm. This paper studies the expression of GPX5 in the epididymis of sheep at different ages. The results are as follows: 1. by real-time quantitative fluorescence quantitative PCR, the GPX5 gene was detected at different ages. The relative expression of the epididymis, testis and vas deferens showed that the GPX5 gene was almost not expressed in the epididymis of young (2-3 months old) lambs, testis and vas deferens, while the expression of GPX5 gene in the epididymal head of the young (6-12 month old) sheep was significantly higher than that of the epididymis and epididymal tail (P0.01), and was almost not expressed in the spermatic duct and testis; in adulthood (2-3) The epididymal head of sheep was significantly higher than the epididymal body and epididymal tail (P0.05), but it was significantly lower than the expression of.GPX5 in the epididymis of the young sheep. There was no significant difference in the expression of the testis and spermatic duct in the sheep of different ages..2. was used to detect the expression of GPX5 protein in the epididymis and testis of different age sheep by Western blot. The results showed that the lambs were in the lambs. GPX5 protein was not detected in epididymis and testis of sheep; expressed in the epididymal head of young sheep, low expression of epididymal body and epididymal tail, not expressed in the testis, a small amount of expression in the epididymal head of adult sheep, epididymal body, epididymal tail and testis almost did not express the use of.3. immunohistochemical method to locate the GPX5 protein in epididymis. The GPX5 protein is mainly expressed in the cytoplasm and the cilium of the epididymal epithelium. The GPX5 protein in the epididymal body and epididymal tail is mainly concentrated in the epididymal cavity and in a small amount of epithelium. And the GPX5 protein of the epididymal tail is mainly secreted from the epididymal epithelium to the epididymal cavity in the caudal epididymal.GPX5 protein, with the transport of sperm in the epididymis. Sperm binding. In a word, the expression of GPX5 in the epididymis of sheep in different ages is different.GPX5 mainly in the young sheep and the epithelia of the epididymis of adult sheep, GPX5 protein secreted into the epididymal cavity with sperm, protecting sperm from the attack of active oxygen (ROS) during the epididymal transport, so as to ensure the normal functioning of the sperm function.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826

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