鸭腺病毒A型Hexon基因克隆与序列分析

发布时间：2018-06-28 06:08

本文选题：鸭腺病毒A型 + Hexon基因　；参考：《中国畜牧兽医》2017年10期

【摘要】：为明确鸭腺病毒A型Hexon基因特征及其在腺病毒科病毒分类中的作用,本研究从前期分离鉴定的一株番鸭源鸭腺病毒A型分离株(JX2016株)中分段扩增获得其Hexon基因编码区。结果发现,鸭腺病毒A型JX2016株Hexon基因编码区为2 733 bp,编码910个氨基酸,与鸭腺病毒A型(FJ12025株)核苷酸同源性最高,达99.8%;与其他分离株Hexon基因核苷酸同源性均在99.4%以上;与鸭腺病毒2型(GR株,未明确分类地位)同源性仅为54.5%;与禽腺病毒A型Phelps株(鸡源)及P29株(鹅源)的核苷酸同源性分别为53.6%和55.2%。遗传进化树分析发现,所有鸭腺病毒A型分离株在遗传进化上均处于相同的亚分支,且与绵羊腺病毒D型(OAV287株)均处于富AT腺病毒属遗传进化分支。但鸭腺病毒2型(GR株)与禽腺病毒A型Phelps株(鸡源)、P29株(鹅源)却处于同一遗传进化分支,均属于禽腺病毒属遗传进化分支。基于Hexon蛋白的遗传进化分析,建议将鸭腺病毒A型重新命名为鸭源富AT腺病毒A型,将鸭腺病毒2型重新命名为鸭源禽腺病毒。
[Abstract]:In order to identify the characteristics of Hexon gene of duck adenovirus A and its role in the classification of adenoviridae, the coding region of Hexon gene was amplified from a strain of Muscovy duck adenovirus A (JX2016 strain). The results showed that the coding region of Hexon gene of duck adenovirus type A JX2016 strain was 2 733 BP, encoding 910 amino acids, the highest nucleotide homology with duck adenovirus type A (FJ12025 strain) was 99.8%, and the nucleotide homology of Hexon gene with other isolates was over 99.4%. The homology was only 54.5% with duck adenovirus type 2 (gr strain), 53.6% with avian adenovirus A Phelps strain (chicken source) and 55.2% with P29 strain (goose source). Genetic phylogenetic tree analysis showed that all duck adenovirus A isolates were in the same subbranch in genetic evolution and were in the genetic evolution branch of AT rich adenovirus with sheep adenovirus type D (OAV287 strain). However, duck adenovirus type 2 (gr strain) and avian adenovirus type A Phelps strain (chicken source) P29 strain (goose source) are in the same genetic evolution branch, both belong to the genetic evolution branch of avian adenovirus. Based on the genetic and evolutionary analysis of Hexon protein, it is suggested to rename duck adenovirus type A to duck AT rich adenovirus type A and duck adenovirus type 2 to duck origin avian adenovirus.
【作者单位】：福建省农业科学院畜牧兽医研究所福建省畜禽疫病防治工程技术研究中心福建省禽病防治重点实验室;
【基金】：国家水禽产业技术体系(CARS-43) 国家自然科学基金(31602068) 福建省农业科学院畜牧兽医研究所基金[MYQJ2015(S)-3] 福建省自然科学基金(2015J01114) 福建省农业科学院“青年科技英才百人计划”专项(YC2015-12)
【分类号】：S852.65

【相似文献】